Author | Pereira, Paula Fabiana Santos | |
Author | Silva, Ana Carolina Alves de Paula e | |
Author | Pimentel, Bruna Natália Alves da Silva | |
Author | Pinatti, Ivo Mateus | |
Author | Simões, Alexandre Zirpoli | |
Author | Vergani, Carlos Eduardo | |
Author | Vieira, Débora Ferreira Barreto | |
Author | Silva, Marcos Alexandre Nunes da | |
Author | Miranda, Milene Dias | |
Author | Monteiro, Maria Eduarda Santos | |
Author | Tucci, Amanda | |
Author | Doñate‑Buendía, Carlos | |
Author | Mínguez‑Vega, Gladys | |
Author | Andrés, Juan | |
Author | Longo, Elson | |
Access date | 2022-06-14T12:43:30Z | |
Available date | 2022-06-14T12:43:30Z | |
Document date | 2022 | |
Citation | PEREIRA, Paula Fabiana Santos et al. Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation. Scientific Reports, v. 12, 8118, p. 1-18, 2022. | pt_BR |
ISSN | 2045-2322 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/53245 | |
Language | eng | pt_BR |
Publisher | Nature | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | SARS‑CoV‑2 | pt_BR |
Subject in Portuguese | COVID-19 | pt_BR |
Subject in Portuguese | Inativação do SARS-CoV-2 | pt_BR |
Subject in Portuguese | Composto quitosana/α-Ag2WO4 | pt_BR |
Subject in Portuguese | Gerado por laser | pt_BR |
Subject in Portuguese | Pandemia | |
Title | Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation | pt_BR |
Type | Article | pt_BR |
DOI | 10.1038/s41598-022-11902-5 | |
Abstract | In the current COVID-19 pandemic, the next generation of innovative materials with enhanced anti-SARS-CoV-2 activity is urgently needed to prevent the spread of this virus within the community. Herein, we report the synthesis of chitosan/α-Ag2WO4 composites synthetized by femtosecond laser irradiation. The antimicrobial activity against Escherichia coli, Methicilin-susceptible Staphylococcus aureus (MSSA), and Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimal bactericidal/fungicidal concentration (MBC/MFC). To assess the biocompatibility of chitosan/α-Ag2WO4 composites in a range involving MIC and MBC/MFC on keratinocytes cells (NOK-si), an alamarBlue™ assay and an MTT assay were carried out. The SARSCoV- 2 virucidal effects was analyzed in Vero E6 cells through viral titer quantified in cell culture
supernatant by PFU/mL assay. Our results showed a very similar antimicrobial activity of chitosan/α- Ag2WO4 3.3 and 6.6, with the last one demonstrating a slightly better action against MSSA. The chitosan/α-Ag2WO4 9.9 showed a wide range of antimicrobial activity (0.49–31.25 μg/mL). The cytotoxicity outcomes by alamarBlue™ revealed that the concentrations of interest (MIC and MBC/ MFC) were considered non-cytotoxic to all composites after 72 h of exposure. The Chitosan/α-Ag2WO4 (CS6.6/α-Ag2WO4) composite reduced the SARS-CoV-2 viral titer quantification up to 80% of the controls. Then, our results suggest that these composites are highly efficient materials to kill bacteria (Escherichia coli, Methicillin-susceptible Staphylococcus aureus, and the yeast strain Candida albicans), in addition to inactivating SARS-CoV-2 by contact, through ROS production. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Departamento de Química. CDMF, LIEC. São Carlos, SP, Brasil / Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain. | pt_BR |
Affilliation | Universidade Estadual de São Paulo. Escola de Odontologia. Departamento de Materiais Dentários e Prótese Dentária. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Universidade Estadual de São Paulo. Escola de Odontologia. Departamento de Materiais Dentários e Prótese Dentária. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain / Universidade Estadual de São Paulo. Faculdade de Engenharia de Guaratinguetá, SP, Brasil. | pt_BR |
Affilliation | Universidade Estadual de São Paulo. Faculdade de Engenharia de Guaratinguetá, SP, Brasil. | pt_BR |
Affilliation | Universidade Estadual de São Paulo. Escola de Odontologia. Departamento de Materiais Dentários e Prótese Dentária. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | GROC UJI, Institute of New Imaging Technologies, Universitat Jaume I, Avda. Sos Baynat sn, 12071 Castellón de la Plana, Spain / Materials Science and Additive Manufacturing, University of Wuppertal, Gaußstr. 20, 42119 Wuppertal, Germany. | pt_BR |
Affilliation | GROC UJI, Institute of New Imaging Technologies, Universitat Jaume I, Avda. Sos Baynat sn, 12071 Castellón de la Plana, Spain. | pt_BR |
Affilliation | Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Departamento de Química. CDMF, LIEC. São Carlos, SP, Brasil. | pt_BR |
Subject | SARS‑CoV‑2 | pt_BR |
Subject | COVID-19 | pt_BR |
Subject | Inactivation of SARS‑CoV‑2 | pt_BR |
Subject | Chitosan/α‑Ag2WO4 composite | pt_BR |
Subject | Second laser irradiation | pt_BR |
Subject | Pandemic | |