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EVALUATION OF HEAT TREATMENT FOR INACTIVATION OF NOROVIRUS GENOGROUP II IN FOODS
Norovírus murino 1
Inativação térmica
Molho de tomate
Carne moída
Comida
Murine norovirus 1
Thermal inactivation
Tomato sauce
Ground meat
Food
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambeintal. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambeintal. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambeintal. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
The effective food processing technology is a key step in eliminating human noroviruses in foods mainly due to their stability
in diverse environmental conditions. The aim of this study was to evaluate the effect of rising temperatures for inactivation
of norovirus genogroup (G) II and murine norovirus 1 in samples of tomato sauce (72–74 °C for 1 min) and ground meat
(100 °C for 30 min). Spiking experiments were carried out in triplicate using TRIzol® reagent method associated with
quantitative polymerase chain reaction (qPCR) TaqMan™ system combined with previous free RNA digestion. Success
rate and efficiency recoveries of both viruses as well limit of detection of a method for each matrix were also conducted.
The heat treatment applied here proved to be efficient to reduce the burden of norovirus GII in a range of 1–4 log10
genomic
copies per gram (percentage ranging from 0.45 to 104.54%) in both matrices. The experiments in this study showed that the
results of norovirus GII and murine norovirus 1 in tomato sauce and ground meat tested during thermal treatments cannot
be generalized to other food matrices, since there may be food-specific protective effects, as the presence of different components,
that can interfere in virus inactivation. Studies using different food matrices reinforce the importance to investigate
viruses’ inactivation thermal processes in foods due to the resistance of these viruses to adverse conditions, contributing to
food security in food virology.
Palabras clave en portugues
Norovírus humano GIINorovírus murino 1
Inativação térmica
Molho de tomate
Carne moída
Comida
Palabras clave en ingles
Human norovirus GIIMurine norovirus 1
Thermal inactivation
Tomato sauce
Ground meat
Food
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