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https://www.arca.fiocruz.br/handle/icict/53671
DIVERSITY OF CRONOBACTER GENUS ISOLATED BETWEEN 1970-2019 ON THE AMERICAN CONTINENT AND GENOTYPED USING MULTI-LOCUS SEQUENCE TYPING
MLST
Variação Genética
Epidemiologia
Patógenos de origem alimentar
Infecções Bacterianas
Author
Affilliation
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Departamento de Controle da Qualidade. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Departamento de Controle da Qualidade. Rio de Janeiro, RJ, Brasil.
Foodmicrobe.com. Nottinghamshire, United Kingdom.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Departamento de Controle da Qualidade. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Departamento de Controle da Qualidade. Rio de Janeiro, RJ, Brasil.
Foodmicrobe.com. Nottinghamshire, United Kingdom.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Departamento de Controle da Qualidade. Rio de Janeiro, RJ, Brasil.
Abstract
This study aimed to evaluate the Cronobacter spp. strains isolated on the American continent and characterized using multi-locus sequence typing (MLST) available in the PubMLST database and current literature. From 465 Cronobacter spp. strains, the majority (n = 267, 57.4%) was from North America, mainly from USA (n = 234) and 198 (42.6%) were from South America, mainly from Brazil (n = 196). A total of 232 (49.9%) were isolated from foods, 102 (21.9%) from environmental, 87 (18.7%) from clinical, 27 (5.8%) from PIF, one from water (0.2%) and 16 (3.5%) from unknown sources. A total of five species were represented: Cronobacter sakazakii (374, 80.4%), Cronobacter malonaticus (41, 8.8%), Cronobacter dublinensis (29, 6.2%),
Cronobacter turicensis (16, 3.5%) and Cronobacter muytjensii (5, 1.1%). The strains with complete MLST profile (n = 345) were assigned to 98 STs, a ratio of 3.5 strain by ST found and the calculated Simpson`s index was 0.93. The strains showed a high diversity and after eBURST analysis, 30 STs (n = 189) formed 12 single and/or double-locus variant clonal complexes (CC). A total of 38 STs (38.7%) were associated with clinical cases of infection, including well established C. sakazakii CC 1, 4, 8 and 83; C. malonaticus ST60, 307, 394 and 440; and C. sakazakii ST 12 and 494.
Keywords in Portuguese
CronobacterMLST
Variação Genética
Epidemiologia
Patógenos de origem alimentar
Infecções Bacterianas
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