Author | Magalhães, Chaiana Froés | |
Author | Siqueira Filho, Ezequias Pessoa de | |
Author | Oliveira, Elaine Alves de | |
Author | Zani, Carlos Leomar | |
Author | Peres, Rodrigo Loreto | |
Author | Santos, Kênia Valéria dos | |
Author | Andrade, Anderson Assunção | |
Access date | 2022-08-01T16:46:41Z | |
Available date | 2022-08-01T16:46:41Z | |
Document date | 2016 | |
Citation | MAGALHÃES, Chaiana Froés et al. Antimicrobial activity of Piper aduncum leaf extracts against the dental plaque bacteria Streptococcus mutans and Streptococcus sanguinis. Journal of Medicinal Plant Research, v. 10, n. 23, p. 331-337, 2016. DOI: 10.5897/JMPR2015.5956 | pt_BR |
ISSN | 1996-0875 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/54114 | |
Sponsorship | FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAIS | pt_BR |
Language | eng | pt_BR |
Publisher | Academic Journals | pt_BR |
Rights | restricted access | pt_BR |
Title | Antimicrobial activity of Piper aduncum leaf extracts against the dental plaque bacteria Streptococcus mutans and Streptococcus sanguinis | pt_BR |
Type | Article | pt_BR |
DOI | 10.5897/JMPR2015.5956 | |
Abstract | Piper aduncum has been widely used for medicinal purposes, and it has also been known to possess antimicrobial activity. Dental plaque is a complex ecosystem that harbors benign and pathogenic bacteria. It is desirable that compounds targeted to treat dental plaque-related diseases should be selective in their action, preserving the benign bacteria and inactivating the pathogenic ones. Thus, the study evaluated the antibacterial activity of P. aduncum leaf extracts against cariogenic (Streptococcus mutans) and health-associated (Streptococcus sanguinis) bacterium. For this, ethanol extracts were obtained by decoction, maceration, Soxhlet or turbo-extraction. The minimum inhibitory concentration (MIC) of the extracts was determined using the broth microdilution method. The influence of extracts on virulence traits of S. mutans was evaluated by the adherence assay to glass surface and by the glycolytic pH drop assay. S. mutans was more susceptible to crude extracts of P. aduncum than S. sanguinis and the highest activity was obtained with the maceration extract (MAC). Thus, MAC was further fractionated by gel permeation chromatography and the most active fraction against S. mutans (MIC of 0.08 mg/mL) had a MIC of 0.62 mg/mL for S. sanguinis. In addition, this fraction inhibited sucrose-dependent adherence of S. mutans and also reduced the level of acid production by this bacterium. The preferential activity of P. aduncum extracts towards S. mutans compared with S. sanguinis, in addition to their ability to inhibit sucrose-dependent adherence and reduce the level of acid production by S. mutans, suggest that this plant may have a potential to prevent dental caries. | pt_BR |
Affilliation | Universidade Vale do Rio Doce. Faculdade de Ciências da Saúde. Laboratório de Pesquisa em Microbiologia. Governador Valadares, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Vale do Rio Doce. Faculdade de Ciências da Saúde. Laboratório de Pesquisa em Microbiologia. Governador Valadares, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Vale do Rio Doce. Faculdade de Ciências da Saúde. Laboratório de Pesquisa em Microbiologia. Governador Valadares, MG, Brazil/Instituto Federal Baiano. Teixeira de Freitas, BA, Brazil. | pt_BR |
Affilliation | Universidade Vale do Rio Doce. Faculdade de Ciências da Saúde. Laboratório de Pesquisa em Microbiologia. Governador Valadares, MG, Brazil/Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia. Vitória, ES, Brazil. | pt_BR |
Affilliation | Universidade Vale do Rio Doce. Faculdade de Ciências da Saúde. Laboratório de Pesquisa em Microbiologia. Governador Valadares, MG, Brazil/Universidade Federal do Triângulo Mineiro. Departamento de Microbiologia, Imunologia e Parasitologia. Disciplina de Microbiologia. Uberaba, MG, Brazil. | pt_BR |
Subject | Plant extracts | pt_BR |
Subject | Piper aduncum | pt_BR |
Subject | antibacterial activity | pt_BR |
Subject | Streptococcus mutans | pt_BR |
Subject | caries prevention. | pt_BR |