Author | Souza, Carolina Senra Alves de | |
Author | Gomes, Luciana Inácia | |
Author | Siqueira, Liliane Maria Vidal | |
Author | Coelho, Paulo Marcos Zech | |
Author | Rabello, Ana Lúcia Teles | |
Author | Oliveira, Edward José de | |
Access date | 2022-10-10T18:38:49Z | |
Available date | 2022-10-10T18:38:49Z | |
Document date | 2018 | |
Citation | SOUZA, Carolina Senra Alves de et al. Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA. BMC Res Notes, v. 11, n. 1, 455, 2018. doi: 10.1186/s13104-018-3571-7. | en_US |
ISSN | 1756-0500 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/55090 | |
Language | eng | en_US |
Publisher | Biomed Central | en_US |
Rights | open access | en_US |
Title | Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA | en_US |
Type | Article | en_US |
DOI | 10.1186/s13104-018-3571-7 | |
Abstract | Objective: We developed a laboratorial platform to release a commercial platform used in the PCR-ELISA for the molecular diagnosis of schistosomiasis mansoni. On following, PCR-ELISA platform laboratorial was evaluated in 206 feces samples collected of individual living in a Brazilian low endemicity area.
Results: The PCR-ELISA laboratorial platform indicated a prevalence rate of 25.2%, which was higher than the Kato-Katz technique (18.4%) and lower than the commercial platform (30.1%). Considering Kato-Katz technique as the reference, there were 97.4% and 91.1% of relative sensitivity and specificity rates, respectively. The laboratorial platform presented good precision, performance diagnostic, and can be used in replacement to the commercial platform for diagnosis of schistosomiasis by PCR-ELISA. | en_US |
Affilliation | Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Biology of the Schistosoma mansoni and its Interaction with Host. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Biology of the Schistosoma mansoni and its Interaction with Host. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
Subject | Diagnosis | en_US |
Subject | Disease control | en_US |
Subject | PCR-ELISA | en_US |
Subject | Schistosoma mansoni | en_US |
Subject | Schistosomiasis | en_US |