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https://www.arca.fiocruz.br/handle/icict/55467
PEPTIDERGIC SIGNALING FROM CLOCK NEURONS REGULATES REPRODUCTIVE DORMANCY IN DROSOPHILA MELANOGASTER
Author
Affilliation
Department of Biology. University of Padova. Padova, Italy.
Department of Biology. University of Padova. Padova, Italy.
Department of Biology. University of Padova. Padova, Italy.
Department of Genetics and Genome Biology. University of Leiceste., Leicester, United Kingdom
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Department of Genetics and Genome Biology. University of Leiceste., Leicester, United Kingdom/Fundação Oswaldo Cruz. Instituto Rene Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patogeno-Vetor. Belo Horizonte, MG, Brazil
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Department of Biology. University of Padova. Padova, Italy.
Department of Genetics and Genome Biology. University of Leiceste., Leicester, United Kingdom.
Department of Genetics and Genome Biology. University of Leiceste., Leicester, United Kingdom.
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Department of Biology. University of Padova. Padova, Italy.
Department of Biology. University of Padova. Padova, Italy.
Department of Biology. University of Padova. Padova, Italy.
Department of Genetics and Genome Biology. University of Leiceste., Leicester, United Kingdom
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Department of Genetics and Genome Biology. University of Leiceste., Leicester, United Kingdom/Fundação Oswaldo Cruz. Instituto Rene Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patogeno-Vetor. Belo Horizonte, MG, Brazil
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Department of Biology. University of Padova. Padova, Italy.
Department of Genetics and Genome Biology. University of Leiceste., Leicester, United Kingdom.
Department of Genetics and Genome Biology. University of Leiceste., Leicester, United Kingdom.
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Department of Biology. University of Padova. Padova, Italy.
Abstract
With the approach of winter, many insects switch to an alternative protective developmental program called diapause. Drosophila melanogaster females overwinter as adults by inducing a reproductive arrest that is characterized by inhibition of ovarian development at previtellogenic stages. The insulin producing cells (IPCs) are key regulators of this process, since they produce and release insulin-like peptides that act as diapause-antagonizing hormones. Here we show that in D. melanogaster two neuropeptides, Pigment Dispersing Factor (PDF) and short Neuropeptide F (sNPF) inhibit reproductive arrest, likely through modulation of the IPCs. In particular, genetic manipulations of the PDF-expressing neurons, which include the sNPF-producing small ventral Lateral Neurons (s-LNvs), modulated the levels of reproductive dormancy, suggesting the involvement of both neuropeptides. We expressed a genetically encoded cAMP sensor in the IPCs and challenged brain explants with synthetic PDF and sNPF. Bath applications of both neuropeptides increased cAMP levels in the IPCs, even more so when they were applied together, suggesting a synergistic effect. Bath application of sNPF additionally increased Ca2+ levels in the IPCs. Our results indicate that PDF and sNPF inhibit reproductive dormancy by maintaining the IPCs in an active state.
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