Author | Robert, Anny Waloski | |
Author | Marcon, Bruna Hilzendeger | |
Author | Angulski, Addeli Bez Batti | |
Author | Martins, Sharon de Toledo | |
Author | Leitolis, Amanda | |
Author | Stimamiglio, Marco Augusto | |
Author | Senegaglia, Alexandra Cristina | |
Author | Correa, Alejandro | |
Author | Alves, Lysangela Ronalte | |
Access date | 2022-11-29T17:33:26Z | |
Available date | 2022-11-29T17:33:26Z | |
Document date | 2022 | |
Citation | ROBERT, Anny Waloski et al. Selective loading and variations in the miRNA profile of extracellular vesicles from endothelial-like cells cultivated under normoxia and hypoxia. Int. J. Mol. Sci., v. 23, n. 10066, p. 1–20, 2022. | en_US |
ISSN | 1422-0067 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/55832 | |
Language | por | en_US |
Publisher | MDPI | en_US |
Rights | open access | en_US |
Subject in Portuguese | miRNA | en_US |
Subject in Portuguese | E-CD133 | en_US |
Title | Selective loading and variations in the miRNA profile of extracellular vesicles from endothelial-like cells cultivated under normoxia and hypoxia | en_US |
Type | Article | en_US |
DOI | https://doi.org/10.3390/ ijms231710066 | |
Abstract | Endothelial-like cells may be obtained from CD133+ mononuclear cells isolated from human umbilical cord blood (hUCB) and expanded using endothelial-inducing medium (E-CD133 cells). Their use in regenerative medicine has been explored by the potential not only to form vessels but also by the secretion of bioactive elements. Extracellular vesicles (EVs) are prominent messengers of this paracrine activity, transporting bioactive molecules that may guide cellular response under different conditions. Using RNA-Seq, we characterized the miRNA content of EVs derived from E-CD133 cells cultivated under normoxia (N-EVs) and hypoxia (H-EVs) and observed that changing the O2 status led to variations in the selective loading of miRNAs in the EVs. In silico analysis showed that among the targets of differentially loaded miRNAs, there are transcripts involved in pathways related to cell growth and survival, such as FoxO and HIF-1 pathways. The data obtained reinforce the pro-regenerative potential of EVs obtained from E-CD133 cells and shows that fine tuning of their properties may be regulated by culture conditions. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | en_US |
Affilliation | Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil. / Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. / Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. | en_US |
Subject | Extracellular Vesicles | en_US |
Subject | Cell Hypoxia | en_US |
Subject | Endothelial Cells | en_US |
Subject in Spanish | Vesículas Extracelulares | en_US |
Subject in Spanish | Hipoxia de la Célula | en_US |
Subject in Spanish | MicroARNs | en_US |
Subject in Spanish | Células Endoteliales | en_US |
Subject in French | Vésicules extracellulaires | en_US |
Subject in French | Hypoxie cellulaire | en_US |
Subject in French | microARN | en_US |
Subject in French | Cellules endothéliales | en_US |
DeCS | Vesículas Extracelulares | en_US |
DeCS | Hipóxia Celular | en_US |
DeCS | MicroRNAs | en_US |
DeCS | Células Endoteliais | en_US |