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2099-12-31
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CHARACTERIZATION OF AGGLUTINATING ANTIBODIES DETECTED BY THE DIRECT AGGLUTINATION TEST FOR VISCERAL LEISHMANIASIS DIAGNOSIS
Antibody subtypes
Direct agglutination test
Visceral leishmaniasis
Afiliación
Oswaldo Cruz Foundation. Instituto René Rachou. Clinical Research and Public Politics in Infectious and Parasitic Diseases. Belo Horizonte, MG, Brazil/Oswaldo Cruz Foundation. Instituto René Rachou. Functional Genomic of Parasites. Belo Horizonte, MG, Brazil.
Oswaldo Cruz Foundation. Instituto René Rachou. Clinical Research and Public Politics in Infectious and Parasitic Diseases. Belo Horizonte, MG, Brazil/Oswaldo Cruz Foundation. Instituto René Rachou. Functional Genomic of Parasites. Belo Horizonte, MG, Brazil
Oswaldo Cruz Foundation. Instituto René Rachou. Clinical Research and Public Politics in Infectious and Parasitic Diseases. Belo Horizonte, MG, Brazil/Oswaldo Cruz Foundation. Instituto René Rachou. Functional Genomic of Parasites. Belo Horizonte, MG, Brazil.
Oswaldo Cruz Foundation. Instituto René Rachou. Clinical Research and Public Politics in Infectious and Parasitic Diseases. Belo Horizonte, MG, Brazil/Oswaldo Cruz Foundation. Instituto René Rachou. Functional Genomic of Parasites. Belo Horizonte, MG, Brazil
Oswaldo Cruz Foundation. Instituto René Rachou. Clinical Research and Public Politics in Infectious and Parasitic Diseases. Belo Horizonte, MG, Brazil/Oswaldo Cruz Foundation. Instituto René Rachou. Functional Genomic of Parasites. Belo Horizonte, MG, Brazil.
Resumen en ingles
This study aimed to characterize agglutinating antibodies detected by the direct agglutination test (DAT-LPC) for the diagnosis of visceral leishmaniasis (VL). The DAT-LPC antigen/antibodies complex was recovered, washed, and used as antigenic substrate in a modified enzyme-linked immunosorbent assay (modified ELISA), revealed with anti-human IgM, IgG, and IgG subtype conjugates, and in the immunofluorescent antibodies test (IFAT), revealed with anti-human IgG and IgG1 conjugates. IgM antibodies were detected in 50%, IgG and IgG1 in 100%, and IgG3 in 52.8% of the 36 samples from VL patients. IFAT showed that agglutinating IgG and IgG1 antibodies recognized more intensely antigens located in the membrane and kinetoplast of the parasite. No antibodies were detected in the 15 samples from healthy individuals. This study shows for the first time that the antibodies responsible for agglutination in DAT-LPC are mostly of the IgG1 subtype.
Palabras clave en ingles
AntibodiesAntibody subtypes
Direct agglutination test
Visceral leishmaniasis
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