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LEPTOSPIRA INTERROGANS INSOLUBLE FRACTION AS A POTENTIAL ANTIGEN SOURCE FOR LATERAL FLOW IMMUNOCHROMATOGRAPHY
Autor
Afiliación
Universidade Federal Fluminense, Departamento de Biologia Celular e Molecular, Niterói, RJ, Brasil / Universidade Federal Fluminense. Programa de Pós-Graduação em Biotecnologia. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos, Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos, Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Laboratório de Pesquisas sobre o Timo, Rio de Janeiro, RJ, Brasil / Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brasil.
Universidade Federal Fluminense, Departamento de Biologia Celular e Molecular, Niterói, RJ, Brasil / Universidade Federal Fluminense. Programa de Pós-Graduação em Biotecnologia. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos, Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Bio-Manguinhos, Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Laboratório de Pesquisas sobre o Timo, Rio de Janeiro, RJ, Brasil / Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brasil.
Universidade Federal Fluminense, Departamento de Biologia Celular e Molecular, Niterói, RJ, Brasil / Universidade Federal Fluminense. Programa de Pós-Graduação em Biotecnologia. Niterói, RJ, Brasil.
Resumen en ingles
BACKGROUND Leptospirosis is an emerging zoonosis that affects humans and animals. Immunochromatography rapid test is widely
used for early diagnosis of leptospirosis, but with low sensitivity and specificity.
OBJECTIVES To evaluate Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography.
METHODS Insoluble fraction derived from the crude bacterial extract was obtained by serial centrifugation. The polypeptide profile was
determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Immune reactivity of this fraction was assessed
by Western Blotting and lateral flow immunochromatography (LFI). It was tested 160 microagglutination test (MAT)-positive sera from
patients in the acute phase, 100 MAT-negative sera from patients with acute febrile illness, and 45 patients with other infectious diseases.
FINDINGS There was a predominance of low molecular mass-polypeptide bands, ranging from 2 to 37 kDa. The antibody reactivity
of theses polypeptides was found to range from 13-50%, especially between 10 and 38 kDa. Among MAT-positive sera of patients with
leptospirosis in the acute phase, 97% were also positive in LFI, indicating high sensitivity. Among MAT-negative sera, all were negative in
LFI, indicating high specificity. Only 2% of cross-reactivity was detected.
CONCLUSION The insoluble fraction can be a valuable antigen source for development of point-of-care diagnosis test for leptospirosis.
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