Author | Coelho, Larissa D. | |
Author | Souza, Mirna M. D. | |
Author | Cassali, Geovanni D. | |
Author | Silva, Raphaela A. | |
Author | Paiva, Maria J. N. | |
Author | Barros, André L. B. | |
Author | Teixeira, Eliane Morais | |
Author | Silveira, Josianne N. | |
Author | Coelho, Paulo Marcos Zech | |
Author | Aguiar, Marta M. G. | |
Author | Oliveira, Mônica C. | |
Access date | 2023-04-19T18:37:21Z | |
Available date | 2023-04-19T18:37:21Z | |
Document date | 2023 | |
Citation | COELHO, Larissa D. et al. Emetic Tartar-Loaded Liposomes as a New Strategy for Leishmaniasis Treatment. Pharmaceutics, v. 15, n. 3, p. 1-14, Mar. 2023. | en_US |
ISSN | 1999-4923 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/57897 | |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | en_US |
Title | Emetic Tartar-Loaded Liposomes as a New Strategy for Leishmaniasis Treatment | en_US |
Type | Article | en_US |
DOI | 10.3390/pharmaceutics15030904 | |
Abstract | Emetic tartar (ET), was used in the treatment of leishmaniasis but its use was discontinued due to its low therapeutic index. Liposomes have been shown to be a promising strategy for delivery of bioactive substances in the region of interest, in order to reduce and/or eliminate undesirable effects. In the present study, liposomes containing ET were prepared and characterized to evaluate acute toxicity as well as their leishmanicidal action using BALB/c mice with an inoculum of Leishmania (Leishmania) infantum. Liposomes were composed of egg phosphatidylcholine and 3ß-[N-(N',N'-dimethylaminoethane)-carbamoyl]cholesterol, with an average diameter of 200 nm, zeta potential of +18 mV, and ET encapsulated into liposomes at a concentration near 2 g/L. Healthy mice were treated with ET or liposome containing ET (Lip-ET) in a single dose of 16 mg/kg of Sb3+ intravenously and observed for 14 days. The death of two animals in the ET-treated group and no deaths in the Lip-ET-treated group was observed. Higher hepatic and cardiac toxicity were observed in animals treated with ET when compared to animals treated with Lip-ET, blank liposomes (Blank-Lip) and PBS. The study of antileishmanial efficacy was conducted by intraperitoneal administration of Lip-ET, for ten consecutive days. It was observed by limiting dilution that treatments with liposomal formulations containing ET, as well as Glucantime®, led to a significant reduction in parasitic load in spleen and liver (p < 0.05) when compared to the untreated control group. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Produtos Farmacêuticos. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Produtos Farmacêuticos. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Produtos Farmacêuticos. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Produtos Farmacêuticos. Belo Horizonte, MG, Brazil. | en_US |
Subject | Acute toxicity | en_US |
Subject | Antileishmanial efficacy | en_US |
Subject | Emetic tartar | en_US |
Subject | Liposomes | en_US |
Subject | Visceral leishmaniasis | en_US |