Author | Gomes, Shayane Martins Rodrigues | |
Author | Brito, Andréia Carolinne de Souza | |
Author | Manfro, Wânia Ferraz Pereira | |
Author | Ribeiro-Alves, Marcelo | |
Author | Ribeiro, Roberto Stefan de Almeida | |
Author | Cal, Mariana Soares da | |
Author | Lisboa, Vinicius da Cunha | |
Author | Abreu, Daniel Paiva Barros de | |
Author | Castilho, Leda dos Reis | |
Author | Porto, Luís Cristóvão de Moares Sobrino | |
Author | Mafort, Thiago Thomáz | |
Author | Lopes, Agnaldo José | |
Author | Silva, Silvia Amaral Gonçalves da | |
Author | Dutra, Patrícia Maria Lourenço | |
Author | Rodrigues, Luciana Silva | |
Access date | 2023-06-13T01:03:07Z | |
Available date | 2023-06-13T01:03:07Z | |
Document date | 2023 | |
Citation | GOMES, Shayane Martins Rodrigues et al. High levels of pro-inflammatory SARS-CoV-2-specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients. PLoS One, v. 18, n. 4, p. 1-20, Apr. 2023. | en_US |
ISSN | 1932-6203 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/58995 | |
Sponsorship | This work was supported by the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), award number E-26/211.155/2019. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en_US |
Language | eng | en_US |
Publisher | Public Library of Science | en_US |
Rights | open access | en_US |
Title | High levels of pro-inflammatory SARS-CoV-2- specific biomarkers revealed by in vitro whole blood cytokine release assay (CRA) in recovered and long-COVID-19 patients | en_US |
Type | Article | en_US |
DOI | 10.1371/journal.pone.0283983 | |
Abstract | Background: Cytokines induced by SARS-CoV-2 infection play a crucial role in the pathophysiology of COVID-19 and hyperinflammatory responses have been associated with poor clinical outcomes, with progression to severe conditions or long-term subacute complications named as long-COVID-19. Methods: In this cross-sectional study, we aimed to evaluate a set of antigen-specific inflammatory cytokines in blood from recovered COVID-19 individuals or who suffered a post-acute phase of SARS-CoV-2 infection compared to healthy individuals with no history of COVID-19 exposition or infection. Interferon-gamma (IFN-γ), IFN-γ-induced protein 10 (IP-10), tumor necrosis factor (TNF), IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, and IL-17A were quantified by multiplex cytometric bead assay and enzyme-linked immunosorbent assay after stimulation of whole blood with recombinant Spike protein from SARS-CoV-2. Additionally, all participants have evaluated for anti-(S) protein-specific IgG antibodies. Clinical specimens were collected within two months of COVID-19 diagnosis. Results: A total of 47 individuals were enrolled in the study, a median age of 43 years (IQR = 14.5), grouped into healthy individuals with no history of infection or exposure to SARS-CoV-2 (unexposed group; N = 21); and patients from the Health Complex of the Rio de Janeiro State University (UERJ), Brazil, who were SARS-CoV-2 positive by RT-PCR (COVID-19 group)-categorized as recovered COVID-19 (N = 11) or long-COVID-19 (N = 15). All COVID-19 patients presented at least one signal or symptom during the first two weeks of infection. Six patients were hospitalized and required invasive mechanical ventilation. Our results showed that COVID-19 patients had significantly higher levels of IFN-γ, TNF, IL-1β, IL-2, IL-6, IL-8, and IP-10 than the unexposed group. The long-COVID-19 group has presented significantly high levels of IL-1β and IL-6 compared to unexposed individuals, but not from recovered COVID-19. A principal-component analysis demonstrated 84.3% of the total variance of inflammatory-SARS-CoV-2 response in the first two components, and it was possible to stratify IL-6, TNF, IL-1β, IL-10, and IL-2 as the top-five cytokines which are candidates to discriminate COVID-19 group (including long-COVID-19 subgroup) and healthy unexposed individuals. Conclusion: We revealed important S protein-specific differential biomarkers in individuals affected by COVID-19, bringing new insights into the inflammatory status or SARS-CoV-2 exposition determination. | en_US |
Affilliation | Rio de Janeiro State University. Parasitology and Immunology. Medical Science Faculty. Department of Microbiology. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Rio de Janeiro State University. Parasitology and Immunology. Medical Science Faculty. Department of Microbiology. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Rio de Janeiro State University. Parasitology and Immunology. Medical Science Faculty. Department of Microbiology. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. National Institute of Infectology Evandro Chagas. Laboratory of Clinical Research on STD/AID. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Rio de Janeiro State University. Department of General Pathology and Laboratories. Laboratory of Immunopathology. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Pedro Ernesto University Hospital. Policlínica Piquet Carneiro. Pulmonary and Tisiology Department. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Rio de Janeiro State University. Department of General Pathology and Laboratories. Laboratory of Immunopathology. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Federal University of Rio de Janeiro. Chemical Engineering Program, Cell Culture Engineering Lab (COPPE). Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Federal University of Rio de Janeiro. Chemical Engineering Program, Cell Culture Engineering Lab (COPPE). Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Rio de Janeiro State University. Department of Histology and Embryology Institute of Biology Roberto Alcantara Gomes. Histocompatibility and Cryopreservation Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Pedro Ernesto University Hospital. Policlínica Piquet Carneiro. Pulmonary and Tisiology Department. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Pedro Ernesto University Hospital. Policlínica Piquet Carneiro. Pulmonary and Tisiology Department. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Rio de Janeiro State University. Parasitology and Immunology. Medical Science Faculty. Department of Microbiology. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Rio de Janeiro State University. Parasitology and Immunology. Medical Science Faculty. Department of Microbiology. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Rio de Janeiro State University. Department of General Pathology and Laboratories. Laboratory of Immunopathology. Rio de Janeiro, RJ, Brazil. | en_US |
Subject | SARS-CoV-2 | en_US |
Subject | COVID-19 | en_US |
Subject | Cytokine Release Assay (CRA) | en_US |
Subject | Long-COVID-19 patients | en_US |
e-ISSN | 1932-6203 | |