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2099-12-31
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MOLECULAR ANALYSIS OF SMFES, A TYROSINE KINASE OF SCHISTOSOMA MANSONI ORTHOLOGOUS TO THE MEMBERS OF THE FES/FPS/FER FAMILY
Fps proto-oncogene protein
Protein tyrosine kinase
Schistosoma mansoni
Signal transduction
Alternative splicing
Autor
Afiliación
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil / Santa Casa de Belo Horizonte. Programa de Pós-Graduação e Pesquisa. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil / Santa Casa de Belo Horizonte. Programa de Pós-Graduação e Pesquisa. Belo Horizonte, MG, Brazil.
Inserm, U 547. Université Lille 2. Institut Pasteur de Lille. IFR 42. Lille, France.
Inserm, U 547. Université Lille 2. Institut Pasteur de Lille. IFR 42. Lille, France.
Inserm, U 547. Université Lille 2. Institut Pasteur de Lille. IFR 42. Lille, France.
Inserm, U 547. Université Lille 2. Institut Pasteur de Lille. IFR 42. Lille, France.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil / Santa Casa de Belo Horizonte. Programa de Pós-Graduação e Pesquisa. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil / Santa Casa de Belo Horizonte. Programa de Pós-Graduação e Pesquisa. Belo Horizonte, MG, Brazil.
Inserm, U 547. Université Lille 2. Institut Pasteur de Lille. IFR 42. Lille, France.
Inserm, U 547. Université Lille 2. Institut Pasteur de Lille. IFR 42. Lille, France.
Inserm, U 547. Université Lille 2. Institut Pasteur de Lille. IFR 42. Lille, France.
Inserm, U 547. Université Lille 2. Institut Pasteur de Lille. IFR 42. Lille, France.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil / Santa Casa de Belo Horizonte. Programa de Pós-Graduação e Pesquisa. Belo Horizonte, MG, Brazil.
Resumen en ingles
A novel protein tyrosine kinase (PTK) was identified in Schistosoma mansoni and designated SmFes. SmFes exhibits the characteristic features of Fes/Fps/Fer (fes, feline sarcoma; fps, Fujinami poultry sarcoma-, fer, fes related) PTKs, containing three coiled-coil regions, an SH2 (Sre-homology-2) and a TK (tyrosine kinase catalytic) domain signature. SmFes is the first gene from the Fes/Fps/Fer family identified in S. mansoni, and is a single copy gene. Phylogenetic analyses revealed that SmFes is most closely related to its invertebrate orthologues. The assembly of the SmFes cDNA and genomic sequences indicated the presence of 18 introns in SmFes. Comparison of its genomic structure with those of human Fps/Fes and Drosophila Fps indicates that intron positions are conserved within the region encoding the kinase domain. Analysis of partial cDNA clones showed the presence of a 9 bp insertion at the 3' end of exon 10, producing two different cDNA populations, pointed as an alternative splicing event. In addition, an allele of SmFes containing a 15 bp insertion was observed in the genomic sequence. Quantitative RT-PCR indicated that the overall transcription level of SmFes is rather low in all parasite developmental stages. Moreover, SmFes mRNA levels decrease progressively after cercarial transformation, consistent with a role for the corresponding protein in the early stages of infection.
Palabras clave en ingles
Fes/Fps/FerFps proto-oncogene protein
Protein tyrosine kinase
Schistosoma mansoni
Signal transduction
Alternative splicing
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