Author | Oliveira, Mariana Villas Bôas Vidal de | |
Author | Alves, Priscila Elias | |
Author | Teixeira, Jessica | |
Author | Monteiro, Mariana Sato de Souza de Bustamante | |
Author | Moreira, Tailane Sant’Anna | |
Author | Ricci Junior, Eduardo | |
Author | Santos, Elisabete Pereira dos | |
Author | Santos-Oliveira, Ralph | |
Author | Marins, Rita de Cássia Elias Estrela | |
Author | Freitas, Zaida Maria Faria de | |
Access date | 2023-12-11T22:36:35Z | |
Available date | 2023-12-11T22:36:35Z | |
Document date | 2023 | |
Citation | OLIVEIRA, Mariana Villas Bôas Vidal de et al. Development, physicochemical evaluation, and in vivo permeation studies of topical formulations containing 0.1% tacrolimus. Brazilian Journal of Pharmaceutical Sciences, n. 59, p. 1-14, 2023. | en_US |
ISSN | 2175-9790 | |
URI | https://www.arca.fiocruz.br/handle/icict/61707 | |
Language | eng | en_US |
Publisher | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas | en_US |
Rights | open access | en_US |
Title | Development, physicochemical evaluation, and in vivo permeation studies of topical formulations containing 0.1% tacrolimus | en_US |
Type | Article | en_US |
DOI | 10.1590/s2175-97902023e201150 | |
Abstract | The objective of this paper was to develop and evaluate two semi-solid pharmaceutical forms containing 0.1% tacrolimus: cream (CRT01) and gel (GLT01). For the evaluation of physicochemical stability, at times 0, 30, 60 and 90 days, at 23°C and at 40°C, High Performance Liquid Chromatography coupled with a Diode Array Detector (HPLC-DAD) was employed. This method was developed and validated for tacrolimus quantification. The occlusivity test and skin permeation assay were also performed, using an animal model (Wistar rats), and the CRT01 and GLT01 were compared to the 0.1% tacrolimus ointment (PFU01) obtained from the University Pharmacy, Federal University of Rio de Janeiro, Brazil. CRT01 and GLT01 presented a homogeneous aspect and consistency adequate for topical products, along with
sensory characteristics above PFU01. They also presented adequate physicochemical stability for 90 days and a lower occlusive effect than PFU01 (p<0.05). CRT01 showed greater affinity for the skin when compared to PFU01 and GLT01, with low systemic absorption. The CRT01 semi solid formulation was considered the most adequate one to treat patients with atopic dermatitis or other dermatologic inflammatory diseases, promoting rational use of tacrolimus. | en_US |
Affilliation | Federal University of Rio de Janeiro. Health Sciences Center. Medicine Department. Pharmacy School. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Federal University of Rio de Janeiro. Health Sciences Center. Medicine Department. Pharmacy School. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Federal University of Rio de Janeiro. Health Sciences Center. Medicine Department. Pharmacy School. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Federal University of Rio de Janeiro. Health Sciences Center. Medicine Department. Pharmacy School. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Federal University of Rio de Janeiro. Health Sciences Center. Medicine Department. Pharmacy School. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Federal University of Rio de Janeiro. Health Sciences Center. Medicine Department. Pharmacy School. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Federal University of Rio de Janeiro. Health Sciences Center. Medicine Department. Pharmacy School. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Nuclear Engineering Institute. Laboratory of Nanoradiopharmaceuticals and Synthesis of Novel Radiopharmaceuticals. Rio de Janeiro, RJ, Brazil / Rio de Janeiro State University. Laboratory of Nanoradiopharmacy and Radiopharmacy. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Federal University of Rio de Janeiro. Health Sciences Center. Medicine Department. Pharmacy School. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Federal University of Rio de Janeiro. Health Sciences Center. Medicine Department. Pharmacy School. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Semi-solid pharmaceutical forms | en_US |
Subject | Analytical methodology | en_US |
Subject | Occlusivity test | en_US |
Subject | In vivo permeation | en_US |
Subject | Tacrolimus | en_US |
e-ISSN | 2175-9790 | |