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https://www.arca.fiocruz.br/handle/icict/61715
NDM-1-ENCODING PLASMID IN ACINETOBACTER CHENGDUENSIS ISOLATED FROM COASTAL WATER
Author
Affilliation
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Investigação em Microbiologia Médica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Universidade Federal da Paraíba. Departamento de Ciências Farmacêuticas. João Pessoa, PB, Brasil.
Instituto Nacional de Pesquisa em Resistência a Antimicrobianos. Hospital de Clínicas de Porto Alegre. Rio Grande do Sul, RS, Brazil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Investigação em Microbiologia Médica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
Universidade Federal da Paraíba. Departamento de Ciências Farmacêuticas. João Pessoa, PB, Brasil.
Instituto Nacional de Pesquisa em Resistência a Antimicrobianos. Hospital de Clínicas de Porto Alegre. Rio Grande do Sul, RS, Brazil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Investigação em Microbiologia Médica. Rio de Janeiro, RJ, Brasil.
Abstract
Background: Acinetobacter spp. may cause difficult-to-treat nosocomial infections due to acquisition of carbapenemases, including New Delhi metallo-β-lactamase (NDM). This genus has been pointed out as a possible actor in the early dissemination of blaNDM, and this gene has been documented in a variety of species.
Objective: Here we describe an Acinetobacter chengduensis (isolate FL51) carrying blaNDM recovered from coastal water in Brazil.
Methods: In vitro techniques included antimicrobial susceptibility and minimum inhibitory concentration tests, PCR, plasmid profile and matting-out/transformation assays. In silico approaches comprised comparative genomic analyses using appropriate databases.
Results: FL51 grew at room temperature in a variety of culture media, excluding MacConkey. It showed resistance to all beta-lactams tested and to ciprofloxacin. blaNDM-1 was identified, and a single replicon was observed in plasmid profile. In silico DNA hybridization revealed Acinetobacter FL51 as being Acinetobacter chengduensis. blaNDM-1 was flanked upstream by ISAba14-aphA6-ISAba125 and downstream by bleMBL-trpF-Δtat, inserted in a 41,068 bp non typeable plasmid named pNDM-FL51. This replicon showed high coverage and identity with other sequences present in plasmids deposited on the GenBank database, recovered almost exclusively from Acinetobacter spp., associated with hospital settings and animal sources.
Conclusion: We described a recently described environmental Acinetobacter species carrying a plasmid-borne blaNDM associated with a Tn125-like structure. Our findings suggest that replicon may play an important role in blaNDM dissemination among distinct settings within this genus and may support the theory of blaNDM emergence from an environmental Acinetobacter.
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