Author | Almeida, Marcos Cerqueira de | |
Author | Silva, Alan C | |
Author | Barral, Aldina Maria Prado | |
Author | Barral Netto, Manoel | |
Access date | 2013-01-22T21:12:43Z | |
Available date | 2013-01-22T21:12:43Z | |
Document date | 2000 | |
Citation | ALMEIDA, M. C. de et al. A simple method for human peripheral blood monocyte isolation. Memórias do Instituto Oswaldo Cruz, v. 95, n. 2, p. 221-223, mar.-apr. 2000. | pt_BR |
ISSN | 0074-0276 | |
URI | https://www.arca.fiocruz.br/handle/icict/6232 | |
Language | eng | pt_BR |
Rights | open access | |
Title | A simple method for human peripheral blood monocyte isolation | pt_BR |
Type | Article | pt_BR |
Abstract | We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes. | pt_BR |
Affilliation | Universidade de Brasília. Faculdade de Ciências Sociais. Departamento de Patologia. Brasília, DF, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Subject | Monocytes | pt_BR |
Subject | Percoll | pt_BR |
Subject | Gradient | pt_BR |
Subject | Isolation | pt_BR |
DeCS | Separação Celular/métodos | pt_BR |
DeCS | Centrifugação com Gradiente de | pt_BR |
DeCS | Concentração/métodos | pt_BR |
DeCS | Monócitos | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Research Support, Non-U.S. Gov't | pt_BR |
DeCS | Research Support, U.S. Gov't, P.H.S. | pt_BR |