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Autor | Guimaraes, Lays Cordeiro | |
Autor | Costa, Pedro Augusto Carvalho | |
Autor | Scalzo Júnior, Sérgio Ricardo Aluotto | |
Autor | Ferreira, Heloísa Athaydes Seabra | |
Autor | Braga, Ana Carolina Soares | |
Autor | Oliveira, Leonardo Camilo de | |
Autor | Figueiredo, Maria Marta | |
Autor | Shepherd, Sarah | |
Autor | Hamilton, Alex | |
Autor | Queiroz-Junior, Celso Martins | |
Autor | Silva, Walison Nunes da | |
Autor | Silva, Natália Jordana Alves da | |
Autor | Alves, Marco Túllio Rodrigues | |
Autor | Santos, Anderson Kenedy | |
Autor | Faria, Kevin Kelton Santos de | |
Autor | Marim, Fernanda Martins | |
Autor | Fukumasu, Heidge | |
Autor | Birbrair, Alexander | |
Autor | Carvalho, Andréa Teixeira de | |
Autor | Aguiar, Renato Santana de | |
Autor | Mitchell, Michael J | |
Autor | Teixeira, Mauro Martins | |
Autor | Costa, Vivian Vasconcelos | |
Autor | Frezard, Frederic | |
Autor | Guimaraes, Pedro Pires Goulart | |
Fecha de acceso | 2024-02-20T15:24:51Z | |
Fecha de disponibilización | 2024-02-20T15:24:51Z | |
Fecha de publicación | 2024 | |
Referencia | GUIMARAES, Lays Cordeiro et al. Nanoparticle-based DNA vaccine protects against SARS-CoV-2 variants in female preclinical models. Nat Commun, v. 15, n. 1, p. 5900, 2024. doi.org/10.1038/s41467-024-44830-1. | en_US |
ISSN | 2041-1723 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/62681 | |
Idioma | eng | en_US |
Editor | Nature Pub. Group | en_US |
Derechos de autor | restricted access | en_US |
Título | Nanoparticle-based DNA vaccine protects against SARS-CoV-2 variants in female preclinical models. | en_US |
Tipo del documento | Article | en_US |
Resumen en Inglés | A safe and effective vaccine with long-term protection against SARS-CoV-2 variants of concern (VOCs) is a global health priority. Here, we develop lipid nanoparticles (LNPs) to provide safe and effective delivery of plasmid DNA (pDNA) and show protection against VOCs in female small animal models. Using a library of LNPs encapsulating unique barcoded DNA (b-DNA), we screen for b-DNA delivery after intramuscular administration. The top-performing LNPs are further tested for their capacity of pDNA uptake in antigen-presenting cells in vitro. The lead LNP is used to encapsulate pDNA encoding the HexaPro version of SARS-CoV-2 spike (LNP-HPS) and immunogenicity and protection is tested in vivo. LNP-HPS elicit a robust protective effect against SARS-CoV-2 Gamma (P.1), correlating with reduced lethality, decreased viral load in the lungs and reduced lung damage. LNP-HPS induce potent humoral and T cell responses against P.1, and generate high levels of neutralizing antibodies against P.1 and Omicron (B.1.1.529). Our findings indicate that the protective efficacy and immunogenicity elicited by LNP-HPS are comparable to those achieved by the approved COVID-19 vaccine from Biontech/Pfizer in animal models. Together, these findings suggest that LNP-HPS hold great promise as a vaccine candidate against VOCs. | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Biochemistry and Immunology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | State University of Minas Gerais. Divinopolis, MG, Brazil. | en_US |
Afiliación | Department of Bioengineering. University of Pennsylvania. Philadelphia, PA, USA. | en_US |
Afiliación | Department of Bioengineering. University of Pennsylvania. Philadelphia, PA, USA. | en_US |
Afiliación | Department of Morphology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Genetics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Animal Science and Food Engineering. University of São Paulo. Pirassununga, SP, Brazil. | en_US |
Afiliación | Department of Dermatology. University of Wisconsin-Madison. Madison, WI, USA. | en_US |
Afiliación | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Genetics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Bioengineering. University of Pennsylvania. Philadelphia, PA, USA. | en_US |
Afiliación | Department of Biochemistry and Immunology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Morphology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Afiliación | Department of Physiology and Biophysics. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
Palavras clave en Inglês | Animals | en_US |
Palavras clave en Inglês | COVID-19/prevention & control | en_US |
Palavras clave en Inglês | DNA, B-Form | en_US |
Palavras clave en Inglês | Nanovaccines | en_US |
Palavras clave en Inglês | SARS-CoV-2/genetics | en_US |
Palavras clave en Inglês | Vaccines, DNA/genetics | en_US |
Palavras clave en Inglês | COVID-19 Vaccines | en_US |
Fecha de embargo | 2025-12-31 |