Author | Pato, Tânia Pinheiro | |
Author | Barbosa, Antonio de Pádua R. | |
Author | Silva Junior, José Godinho da | |
Access date | 2024-03-14T12:04:14Z | |
Available date | 2024-03-14T12:04:14Z | |
Document date | 2006 | |
Citation | PATO, Tânia Pinheiro; BARBOSA, Antônio de Pádua R.; SILVA JÚNIOR, José Godinho da. Purification of capsular polysaccharide from Neisseria meningitidis serogroup C by liquid chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, v. 832, n. 2, p. 262-267, Mar. 2006. | en_US |
ISSN | 1570-0232 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/63037 | |
Language | eng | en_US |
Publisher | Elsevier | en_US |
Rights | restricted access | en_US |
Title | Purification of capsular polysaccharide from Neisseria meningitidis serogroup C by liquid chromatography | en_US |
Type | Article | en_US |
DOI | 10.1016/j.jchromb.2006.01.008 | |
Abstract | Neisseria meningitidis serogroup C capsular polysaccharide (MenCPS) is an important antigen against meningococcal infection. This paper describes a new purification methodology employing liquid chromatography that resulted in a polysaccharide showing the characteristics recommended by the World Health Organization for vaccine purposes. In this method, steps of the traditional procedure that yield low recovery and use toxic materials were modified. The present process consists in the following steps: (1) continuous flow centrifugation of the culture for removal of the cells; (2) supernatant concentration by tangential filtration (100 kDa cutoff); (3) addition of 0.5% DOC, heating to 55 °C during 30 min and tangential filtration (100 kDa cutoff); (4) anion exchange chromatography (Source 15Q) and (5) size exclusion chromatography (Sepharose CL-4B). The polysaccharide C fraction obtained in that way was dialyzed and freeze-dried. The structural identity of the polysaccharide was demonstrated by 1H-NMR spectrometry. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Departamento de Vacinas Bacterianas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Departamento de Vacinas Bacterianas. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil / Universidade Federal Rural do Rio de Janeiro. Instituto de Ciências Exatas. Departamento de Química. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Neisseria meningitidis | en_US |
Subject | Capsular polysaccharide | en_US |
Subject | Purification by liquid chromatography | en_US |
Embargo date | 2030-12-31 | |