| Author | Silva, Esdras Matheus Gomes da | |
| Author | Carvalho, Juliana de Saldanha da Gama Fischer | |
| Author | Souza, Isadora de Lourdes Signorini | |
| Author | Andrade, Amanda Caroline Camillo | |
| Author | Souza, Leonardo de Castro e | |
| Author | Andrade, Marcos Kaoann de | |
| Author | Carvalho, Paulo Costa | |
| Author | Souza, Ricardo Lehtonen Rodrigues | |
| Author | Vital, Maria Aparecida Barbato Frazão | |
| Author | Passetti, Fabio | |
| Access date | 2024-04-09T11:43:15Z | |
| Available date | 2024-04-09T11:43:15Z | |
| Document date | 2024 | |
| Citation | SILVA, Esdras Matheus Gomes da et al. Proteomic analysis of a rat streptozotocin model shows dysregulated biological pathways implicated in Alzheimer's disease. International Journal of Molecular Sciences, v. 25, n. 5, p. 1-15, 28 Feb. 2024. | en_US |
| ISSN | 1661-6596 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/63452 | |
| Sponsorship | This work was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001, Fundação Oswaldo Cruz—Fiocruz, Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ, grant 210.015/2018 MCTI/CNPq/CAPES/FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grants 308697/2019-7, 442421/2019-2, 436459/2018-3, 310299/2022-5, 404226/2023-0, and 440412/2022-6). | en_US |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Title | Proteomic analysis of a rat streptozotocin model shows dysregulated biological pathways implicated in Alzheimer's disease | en_US |
| Type | Article | en_US |
| DOI | 10.3390/ijms25052772 | |
| Abstract | Alzheimer’s Disease (AD) is an age-related neurodegenerative disorder characterized by progressive memory loss and cognitive impairment, affecting 35 million individuals worldwide. Intracerebroventricular (ICV) injection of low to moderate doses of streptozotocin (STZ) in adult male Wistar rats can reproduce classical physiopathological hallmarks of AD. This biological model is known as ICV-STZ. Most studies are focused on the description of behavioral and morphological aspects of the ICV-STZ model. However, knowledge regarding the molecular aspects of the ICV-STZ model is still incipient. Therefore, this work is a first attempt to provide a wide proteome description of the ICV-STZ model based on mass spectrometry (MS). To achieve that, samples from the prefrontal cortex (PFC) and hippocampus (HPC) of the ICV-STZ model and control (wild-type) were used. Differential protein abundance, pathway, and network analysis were performed based on the protein identification and quantification of the samples. Our analysis revealed dysregulated biological pathways implicated in the early stages of late-onset Alzheimer’s disease (LOAD), based on differentially abundant proteins (DAPs). Some of these DAPs had their mRNA expression further investigated through qRT-PCR. Our results shed light on the AD onset and demonstrate the ICV-STZ as a valid model for LOAD proteome description. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Centro Politécnico. Setor de Ciências Biológicas. Departamento de Genética. Laboratório de Polimorfismos e Ligação. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Centro Politécnico. Setor de Ciências Biológicas. Departamento de Farmacologia. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Centro Politécnico. Setor de Ciências Biológicas. Departamento de Farmacologia. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Centro Politécnico. Setor de Ciências Biológicas. Departamento de Genética. Laboratório de Polimorfismos e Ligação. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal do Paraná. Centro Politécnico. Setor de Ciências Biológicas. Departamento de Farmacologia. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Subject | Mass spectrometry | en_US |
| Subject | qRT-PCR | en_US |
| Subject | Pre-frontal cortex | en_US |
| Subject | Hippocampus | en_US |
| Subject | Mitochondrial membrane | en_US |
| Subject | Ribosome | en_US |
| Subject | Insulin secretion | en_US |
| Subject | Chaperone complex | en_US |
| e-ISSN | 1422-0067 | |
