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https://www.arca.fiocruz.br/handle/icict/64085
AVALIAÇÃO DO POTENCIAL LARVICIDA DE NANOPARTÍCULAS DE PRATA SINTETIZADAS A PARTIR DE ALGAS MARINHAS DO LITORAL PERNAMBUCANO CONTRA AEDES AEGYPTI
Aedes
Inseticidas
Nanopartículas Metálicas
patogen
Controle de Mosquitos
Mosquitos Vetores
Inseticidas
Química Verde
Extratos Vegetais
Folhas de Planta
Prata
Larva
Testes de Sensibilidade Microbiana
Spectroscopy, Fourier Transform Infrared
metodos
Espectrometria de Massas
Difração de Raios X
Microscopia de Força Atômica
Cromatografia Líquida de Alta Pressão
Assunção, Matheus Alves Siqueira de | Date Issued:
2024
Advisor
Co-advisor
Comittee Member
Affilliation
Universidade Federal de Pernambuco. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Abstract in Portuguese
Aedes aegypti é o principal mosquito transmissor de arboviroses, possuindo comportamento antropofílico. A nanotecnologia verde tem sido identificada como um método promissor para o biocontrole de mosquitos vetores utilizando biomoléculas, extraídas de fontes naturais para a produção de nanopartículas metálicas. O principal objetivo do presente estudo foi o desenvolvimento de nanopartículas de prata sintetizadas a partir de extratos de algas marinhas com atividade biocida para controle vetorial do Ae. aegypti. As algas foram coletadas na praia de Serrambi, Ipojuca/ PE, secas e utilizadas para a preparação dos extratos brutos aquosos e hidroalcóolicos. As nanopartículas de prata (AgNPs) foram preparadas por síntese verde utilizando o nitrato de prata como precursor e os extratos das algas como agentes redutores e estabilizadores. Os extratos brutos foram caracterizados por FTIR, GC-MS e HPLC-PDAD e as AgNPs por Uv-visível, DLS, Potencial Zeta, DCS, MET, DRX, FTIR, MEV-EDS e DSC. A toxicidade das AgNPs contra larvas de Ae. aegypti e os efeitos na atividade da acetilcolinesterase (AChE), bem como contra larvas de Danio rerio foram avaliadas. As AgNPs, apresentaram picos de Uv-vis entre 400 e 450nm, tamanho entre 198,4-323,0 nm (DLS), PdI entre 0,261-0,470 e potencial zeta entre -38,6 e -47,8. Pela técnica de DCS se observou a presença nanopartículas com tamanhos variando de 11,08-343,22 nm, as quais se apresentaram tanto isoladas quanto agregadas. As imagens obtidas através de Microscopia eletrônica de Transmissão confirmam a escala nanométrica com nanopartículas de tamanhos inferiores à 100 nm. Ademais, a partir das análises de DRX, foi possivel se compreender a natureza cristalina das AgNPs, demonstrando um perfil de tamanho médio variando entre 34,6-86,17nm. Pela análise por FTIR foi possível demonstrar que as biomoléculas presentes nos extratos também estão presentes na redução e estabilização das AgNPs, bem como em seu capeamento. As análises por MEV-EDS demonstraram picos característicos de Ag em 3 keV devido à RPS característica das AgNPs. Pelas análises de DSC foi possível demonstrar a presença de picos endotérmicos e exotérmico relacionados à perda de água, quebra térmica e cristalinidade das AgNps, bem como a dessorções das biomoléculas responsáveis pela redução do sal de prata. As AgNPs demonstraram toxicidade contra larvas de Ae. aegypti com LC50 variando entre 0.50-1.15 ppm e LC90 entra 0.81-2.24ppm, além da variação da presença e atividade da AChE. As AgNPs foram tóxicas para a espécie não-alvo Danio rerio, com LC50 variando entre 0.10-0.29ppm. A síntese biológica rápida de nanopartículas de prata usando extrato de algas marinhas fornece uma rota ecológica, simples e econômica para a síntese de nanopartículas. Os extratos das algas selecionadas foram efetivos na formação das AgNPs, apresentando capacidade redutora e estabilizadora. Apesar da toxicidade em espécies não-alvo, as atividades larvicidas contra larvas de Ae. aegypti, demonstram um avanço importante no desenvolvimento de possíveis candidatos para a substituição de biocidas já comercializados tóxicos para a saúde humana e para o meio-ambiente .
Abstract
Aedes aegypti is the primary mosquito vector of arboviruses, exhibiting anthropophilic behavior. Green nanotechnology has been recognized as a promising method for the biocontrol of vector mosquitoes using biomolecules extracted from natural sources for the production of metallic nanoparticles. The main objective of the present study was the development of silver nanoparticles synthesized from extracts of seaweed with biocidal activity for vector control of Ae. aegypti. Seaweeds were collected at Serrambi Beach, Ipojuca/PE, dried, and used for the preparation of aqueous and hydroalcoholic crude extracts. Silver nanoparticles (AgNPs) were synthesized through green synthesis using silver nitrate as a precursor and seaweeds extracts as reducing and stabilizing agents. The crude extracts were characterized by FTIR, GC-MS, and HPLC-PDA, while the AgNPs were characterized by UV-visible spectroscopy, DLS (Dynamic Light Scattering), Zeta potential, DCS (Differential Centrifugal Sedimentation), TEM (Transmission Electron Microscopy), XRD (X-ray Diffraction), FTIR, SEM-EDS (Scanning Electron Microscopy with Energy Dispersive Spectroscopy), and DSC (Differential Scanning Calorimetry). The toxicity of AgNPs against Ae. aegypti larvae, their effects on acetylcholinesterase (AChE) activity, as well as their impact on Danio rerio larvae, were assessed. The AgNPs exhibited UV-visible peaks between 400 and 450 nm, sizes ranging from 198.4 to 323.0 nm (DLS), PdI (Polydispersity Index) between 0.261 and 0.470, and zeta potential between -38.6 and -47.8. DSC analysis revealed the presence of nanoparticles with sizes ranging from 11.08 to 343.22 nm, appearing both as isolated particles and aggregates. The images obtained through TEM confirm the nanoscale with nanoparticle sizes below 100 nm. Additionally, from XRD analyses, it was possible to understand the crystalline nature of the AgNPs, demonstrating an average size profile ranging from 34.6 to 86.17 nm. FTIR analysis showed that the biomolecules present in the extracts are also involved in the reduction and stabilization of AgNPs, as well as in their capping. SEM-EDS analyses demonstrated characteristic Ag peaks at 3 keV due to the characteristic X-ray emission of AgNPs. DSC analyses revealed the presence of endothermic and exothermic peaks related to water loss, thermal breakdown, and crystallinity of AgNPs, as well as desorption of biomolecules responsible for reducing the silver salt. The AgNPs exhibited toxicity against Ae. aegypti larvae with LC50 ranging between 0.50-1.15 ppm and LC90 between 0.81-2.24 ppm, along with variations in the presence and activity of acetylcholinesterase (AChE). The AgNPs were also toxic to the non-target species Danio rerio, with LC50 ranging between 0.10-0.29 ppm. The rapid biological synthesis of silver nanoparticles using seaweed extract provides an ecological, simple, and cost-effective route for nanoparticle synthesis. The extracts from the selected algae were effective in the formation of AgNPs, displaying both reducing and stabilizing capabilities. Despite the toxicity observed in non-target species, the larvicidal activities against Ae. aegypti larvae represent a significant advancement in the development of potential candidates for replacing commercially available biocides that are toxic to human health and the environment. Aedes aegypti is the primary mosquito vector of arboviruses, exhibiting anthropophilic behavior. Green nanotechnology has been recognized as a promising method for the biocontrol of vector mosquitoes using biomolecules extracted from natural sources for the production of metallic nanoparticles. The main objective of the present study was the development of silver nanoparticles synthesized from extracts of seaweed with biocidal activity for vector control of Ae. aegypti. Seaweeds were collected at Serrambi Beach, Ipojuca/PE, dried, and used for the preparation of aqueous and hydroalcoholic crude extracts. Silver nanoparticles (AgNPs) were synthesized through green synthesis using silver nitrate as a precursor and seaweeds extracts as reducing and stabilizing agents. The crude extracts were characterized by FTIR, GC-MS, and HPLC-PDA, while the AgNPs were characterized by UV-visible spectroscopy, DLS (Dynamic Light Scattering), Zeta potential, DCS (Differential Centrifugal Sedimentation), TEM (Transmission Electron Microscopy), XRD (X-ray Diffraction), FTIR, SEM-EDS (Scanning Electron Microscopy with Energy Dispersive Spectroscopy), and DSC (Differential Scanning Calorimetry). The toxicity of AgNPs against Ae. aegypti larvae, their effects on acetylcholinesterase (AChE) activity, as well as their impact on Danio rerio larvae, were assessed. The AgNPs exhibited UV-visible peaks between 400 and 450 nm, sizes ranging from 198.4 to 323.0 nm (DLS), PdI (Polydispersity Index) between 0.261 and 0.470, and zeta potential between -38.6 and -47.8. DSC analysis revealed the presence of nanoparticles with sizes ranging from 11.08 to 343.22 nm, appearing both as isolated particles and aggregates. The images obtained through TEM confirm the nanoscale with nanoparticle sizes below 100 nm. Additionally, from XRD analyses, it was possible to understand the crystalline nature of the AgNPs, demonstrating an average size profile ranging from 34.6 to 86.17 nm. FTIR analysis showed that the biomolecules present in the extracts are also involved in the reduction and stabilization of AgNPs, as well as in their capping. SEM-EDS analyses demonstrated characteristic Ag peaks at 3 keV due to the characteristic X-ray emission of AgNPs. DSC analyses revealed the presence of endothermic and exothermic peaks related to water loss, thermal breakdown, and crystallinity of AgNPs, as well as desorption of biomolecules responsible for reducing the silver salt. The AgNPs exhibited toxicity against Ae. aegypti larvae with LC50 ranging between 0.50-1.15 ppm and LC90 between 0.81-2.24 ppm, along with variations in the presence and activity of acetylcholinesterase (AChE). The AgNPs were also toxic to the non-target species Danio rerio, with LC50 ranging between 0.10-0.29 ppm. The rapid biological synthesis of silver nanoparticles using seaweed extract provides an ecological, simple, and cost-effective route for nanoparticle synthesis. The extracts from the selected algae were effective in the formation of AgNPs, displaying both reducing and stabilizing capabilities. Despite the toxicity observed in non-target species, the larvicidal activities against Ae. aegypti larvae represent a significant advancement in the development of potential candidates for replacing commercially available biocides that are toxic to human health and the environment .
DeCS
Nanopartículas MetálicasAedes
Inseticidas
Nanopartículas Metálicas
patogen
Controle de Mosquitos
Mosquitos Vetores
Inseticidas
Química Verde
Extratos Vegetais
Folhas de Planta
Prata
Larva
Testes de Sensibilidade Microbiana
Spectroscopy, Fourier Transform Infrared
metodos
Espectrometria de Massas
Difração de Raios X
Microscopia de Força Atômica
Cromatografia Líquida de Alta Pressão
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