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COMPARISON OF DIFFERENT DIRECT DIAGNOSTIC METHODS TO IDENTIFY BABESIA BOVIS AND BABESIA BIGEMINA IN ANIMALS VACCINATED WITH LIVE ATTENUATED PARASITES
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Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Doença de Chagas. Belo Horizonte,MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Doença de Chagas. Belo Horizonte,MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Resumen en ingles
Blood smear examination, flow cytometry, duplex Polymerase Chain Reaction (PCR), and duplex nested PCR (nPCR) were evaluated for detection ofBabesia bigeminaandBabesia bovisinfections in cattle vaccinated with live attenuated strains. Two groups of four cattle were immunized with either B. bigemina (Bi) or B. bovis (Bo). On day 23 post inoculation (PI), Bicattle were vaccinated withB. Bovis (BiBo) and Bocattle were vaccinated withB. Bigemina (BoBi). Babesia bigeminawas first detected by blood smear examination 7.53.5 days PI in the Bi group and 32.21.7 days PI in the Bo Bi group. The first occurrence of B. bovisin blood smears was 8.0 days PI in the Bo group and 36.02.6 days PI in the Bi Bo group. Flow cytometry detected parasitized erythrocytes on day 1.71.5 and 2.21.5 PI in theBiand Bogroups, respectively, but did not discriminate between the twoBabesiaspp. Duplex PCR detected B. bigemina on day 4.00.8 and 26.00.8 PI in theBiand BoBigroups, respectively, and B. bovis on day 4.0 and 25.30.5 PI in the Bo and Bi Bo groups, respectively. The duplex nPCR detected B. bigemina on 3.00.8 and 25.00.0 days PI in the Bi and Bo Bigroups, respectively, and 4.71.7 and 27.76.2 days PI in theBoandBiBogroups, respectively. Duplex nPCR outperformed the other tests in terms of specificity and sensitivity, indicating that it is the most useful method for identifying Babesia spp. in cattle following vaccination.
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