Author | Andrade, Lídia Maria de | |
Author | Geraldo, Jony Marques | |
Author | Gonçalves, Osvaldo Xavier | |
Author | Leite, Miguel T. T. | |
Author | Catarina, Anderson M. | |
Author | Guimarães, Melissa Monteiro | |
Author | Leme, Adriana Franco Paes | |
Author | Yokoo, Sami | |
Author | Machado, Carlos Roberto | |
Author | Rajão, Matheus Andrade | |
Author | Carvalho, Sandhra Maria | |
Author | Gomes, Dawidson Assis | |
Author | Aguiar, Carla Jeane | |
Author | Fagundes, Elaine Maria de Souza | |
Author | Zani, Carlos Leomar | |
Author | Resende, Rodrigo Ribeiro | |
Author | Martins Filho, Olindo Assis | |
Author | Leite, Maria de Fátima | |
Access date | 2014-02-19T16:27:35Z | |
Available date | 2014-02-19T16:27:35Z | |
Document date | 2012 | |
Citation | ANDRADE, Lídia Maria et al. Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy. Journal of Cancer Science & Therapy. 2012, vol.04, pp. 131-139. | pt_BR |
ISSN | 1948-5956 | |
URI | https://www.arca.fiocruz.br/handle/icict/7340 | |
Language | eng | pt_BR |
Publisher | OMICS Group | pt_BR |
Rights | open access | pt_BR |
Title | Nucleoplasmic calcium buffering sensitizes human squamous cell carcinoma to anticancer therapy | pt_BR |
Type | Article | pt_BR |
Abstract | Background: Calcium (Ca2+) signaling within the nucleus is known to play a crucial role in cell proliferation.
The aim of this study was to investigate whether nuclear Ca2+ buffering could improve the antitumor effect of X-rays
therapy on Human Squamous Cell Carcinoma (HSCC).
Methods: For these purpose, we developed an experimental protocol that simulated clinical radiotherapy and
prevented bystander effects of irradiation. HSCC, A431 cell line, was submitted to 10Gy cumulative X-rays therapy
alone (XR Cd 10Gy) or in association with the strategy that selectively buffer nuclear Ca 2+ (Ca 2+ n) signaling.
Results: Upon Ca 2+ n buffering, A431 cell proliferation rate decreased significantly as compared to control. Cell
cycle analysis showed that association of Ca2+ n buffering with XR Cd 10Gy increased the percentage of A431 cells
at G 2 /M and did not increase nuclear/mitochondrial DNA damages. Nonetheless, Ca 2+ n buffering prevented the
increase of the radioresistance-related biomarker ADAM-17 expression and EGFR activation induced by irradiation.
Furthermore, the association therapy almost completely abolished cell survival fraction even using approximately
half of the X-rays cumulative dose
Conclusions: Nuclear Ca 2+ buffering sensitizes human squamous cell carcinoma to X - rays irradiation treatment. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | São Francisco Radiotherapy Institute. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | São Francisco Radiotherapy Institute. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | São Francisco Radiotherapy Institute. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | São Francisco Radiotherapy Institute. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Federal University of Vales do Jequitinhonha and Mucurí. Diamantina,MG, Brazil | pt_BR |
Affilliation | National Laboratory of Biosciences. Campinas, SP, Brazil | pt_BR |
Affilliation | National Laboratory of Biosciences. Campinas, SP, Brazil | pt_BR |
Affilliation | Federal University of Minas Gerais. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Federal University of Minas Gerais. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Federal University of Minas Gerais. School of Engineering. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Federal University of Minas Gerais. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Federal University of Minas Gerais. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Federal University of Minas Gerais. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | René Rachou Research Center. Belo Horizonte,MG, Brazil | pt_BR |
Affilliation | Federal University of Minas Gerais. Department of Biochemistry and Immunology. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. René Rachou Research Center. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Federal University of Minas Gerais. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil/Howard Hughes Medical Institute. USA | pt_BR |
Subject | Human squamous cell carcinoma | pt_BR |
Subject | Nuclear calcium buffering | pt_BR |
Subject | X-rays irradiation | pt_BR |
Subject | A431 cells | pt_BR |
Subject | Head and neck tumor | pt_BR |