Author | Oelemann, Walter Martin Roland | |
Author | Lowndes, Catherine M. | |
Author | Costa, Giovani C. Veríssimo da | |
Author | Morgado, Mariza Gonçalves | |
Author | Castello Branco, Luiz Roberto Ribeiro | |
Author | Grinsztejn, Beatriz | |
Author | Alary, Michel | |
Author | Bastos, Francisco Inácio Pinkusfeld Monteiro | |
Access date | 2010-08-23T16:58:44Z | pt_BR |
Access date | 2010-11-04T14:19:50Z | |
Available date | 2010-11-04T14:19:50Z | |
Available date | 2010-08-23T16:58:44Z | pt_BR |
Document date | 2002 | pt_BR |
Citation | OELEMANN, W. M. R. et al. Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study. Journal of Clinical Microbiology, Washington, v. 40, n. 3, p. 881–885, Mar. 2002. | pt_BR |
ISSN | 1098-660X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/741 | |
Abstract in Portuguese | We evaluated, for the first time in Latin America, the performance of a commercial enzyme immunoassay (EIA) (Calypte Biomedical Corporation, Berkeley, Calif.) that detects human immunodeficiency virus type 1 (HIV-1)-specific antibodies in urine in comparison to standard serological assays (two commercial EIAs and a commercial Western blot [WB] assay). Paired serum and urine specimens were collected from two different groups of Brazilian patients: 225 drug users with unknown HIV status who attended drug treatment centers in Rio de Janeiro, Brazil, and 135 subjects with known HIV status. Patients showing positive results in the serum EIAs and/or in the urine EIA were serologically confirmed by WB assay. For 135 individuals with known HIV status, the urine EIA showed 100% sensitivity (74 positive samples) and 95.1% specificity (58 of 61 negative specimens). For 225 drug users, the test showed 100% sensitivity (2 positive samples) and 98.7% specificity (220 of 223 negative samples) compared to WB-confirmed serological EIA results. Thus, in a total of 360 samples, the urine EIA correctly identified all 76 HIV-positive samples and 278 of 284 negative samples (100% sensitivity
and 97.9% specificity). Detailed analysis of the urine EIA results indicates that an increase of the recommended cutoff value might raise the specificity of the assay without affecting its sensitivity. Our results suggest that the
HIV-1 urine EIA is a good screening test suitable for developing countries like Brazil. However, as for all other HIV screening tests on the market, it is not specific enough to be used as a one-step test and therefore requires confirmation. | pt_BR |
Sponsorship | We thank Maria Inez Linhares de Carvalho, the staff of the Ambulatório da Providência, and the staffs of the Evandro Chagas Hospital
and the drug treatment centers for their unrestricted cooperation.
Diagnostic kits for HIV-1 detection in urine were provided by Calypte Biomedical Corporation.
Financial support for this study was provided by the Brazilian Ministry
of Health and the Brazilian National Research Council (CNPq), and the FIOCRUZ AIDS program (PIAF). M.A. was the recipient of a research scholarship from Fonds de Recherche en Santé de Québec (no. 970097). W.M.R.O., M.G.M, and F.I.B. are recipients of research scholarships from CNPq. | pt_BR |
Language | eng | pt_BR |
Publisher | American Society for Microbiology | pt_BR |
Rights | open access | |
Title | Diagnostic detection of human immunodeficiency virus type 1 antibodies in urine: a Brazilian study | pt_BR |
Type | Article | |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Université Laval. Hôpital du Saint-Sacrement du CHA. Groupe de Recherche en Épidémiologie. Quebec, Canada. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Université Laval. Hôpital du Saint-Sacrement du CHA. Groupe de Recherche en Épidémiologie. Quebec, Canada. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Informação Científica e Tecnológica. Departamento de Informações em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |