Author | Guimarães, Elisalva Teixeira | |
Author | Santos, Luana A | |
Author | Santos, Ricardo Ribeiro dos | |
Author | Teixeira, Mauro Martins | |
Author | dosSantos, Washington Luis Conrado | |
Author | Soares, Milena Botelho Pereira | |
Access date | 2014-05-28T16:49:50Z | |
Available date | 2014-05-28T16:49:50Z | |
Document date | 2006 | |
Citation | GUIMARÃES, E. T. et al. Role of interleukin-4 and prostaglandin E2 in Leishmania amazonensis infection of BALB/c mice. Microbes and Infection, v. 8, n. 5, p. 1219-1226, 2006. | pt_BR |
ISSN | 1286-4579 | |
URI | https://www.arca.fiocruz.br/handle/icict/7661 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | Role of interleukin-4 and prostaglandin E2 in Leishmania amazonensis infection of BALB/c mice. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.micinf.2005.11.011 | |
Abstract | The role of cytokines in Leishmania amazonensis experimental infection has not been as well studied as in Leishmania major infection model. Here we investigated the role of interleukin (IL)-4 and PGE(2) in L. amazonensis infection of susceptible BALB/c mice. IL-4 deficient (-/-) or wild-type (+/+) BALB/c mice were infected with different inocula of L. amazonensis. Two weeks after infection with 5x10(6) promastigotes/footpad, the production of interferon (IFN)-gamma upon L. amazonensis antigen stimulation was significantly higher in lymph node cell cultures of IL-4-/- mice than in IL-4+/+ mice. The levels of anti-leishmania IgG2a antibodies were also significantly higher in serum from IL-4-/- mice. In contrast, the levels of IgG1 antibodies were increased in IL-4+/+ mice and almost undetectable in IL-4-/- mice. Despite the increased Th1 response, lesions of IL-4-/- BALB/c mice progressed similarly to those of IL-4+/+ mice upon infection with the 5x10(6) inoculum. However, IL-4-/- mice developed smaller lesions upon infection with 10(5), 10(4) or 10(3) parasites than IL-4+/+ mice. The resistance of IL-4-/- correlated with higher Th1 response, compared to IL-4+/+ upon infection with 10(4)L. amazonensis. IL-4+/+ mice treated with indomethacin, an inhibitor of PGE(2) synthesis, during the first 3weeks of infection developed smaller lesions and lower parasitic load when compared to the control group. The lesions of indomethacin-treated groups contained mostly macrophages without vacuoles and small or absent necrotic areas. These results indicate that IL-4 and PGE(2) are susceptibility factors to L. amazonensis infection. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Subject | Leishmania amazonensis | pt_BR |
Subject | BALB/c mice | pt_BR |
Subject | Interleukin-4 | pt_BR |
Subject | Prostaglandin E2 | pt_BR |
Subject | Indomethacin | pt_BR |
DeCS | Dinoprostona/metabolismo | pt_BR |
DeCS | Interleucina-4/metabolismo | pt_BR |
DeCS | Leishmania mexicana | pt_BR |
DeCS | Leishmaniose Cutânea/imunologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Anti-Inflamatórios não Esteroides/uso terapêutico | pt_BR |
DeCS | Suscetibilidade a Doenças | pt_BR |
DeCS | Feminino | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Indometacina/uso terapêutico | pt_BR |
DeCS | Interferon gama/biossíntese | pt_BR |
DeCS | Leishmania mexicana/imunologia | pt_BR |
DeCS | Leishmaniose Cutânea/quimioterapia | pt_BR |
DeCS | Leishmaniose Cutânea/fisiopatologia | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Camundongos Endogâmicos BALB C | pt_BR |