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Autor | Santos, Fred Luciano Neves | |
Autor | Gonçalves, Marilda de Souza | |
Autor | Soares, Neci Matos | |
Fecha de acceso | 2014-07-14T14:41:48Z | |
Fecha de disponibilización | 2014-07-14T14:41:48Z | |
Fecha de publicación | 2011 | |
Referencia | SANTOS, F. L. N.; GONÇALVES, M. S.; SOARES, N. M. Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil. Brazilian Journal of Infectious Disease, v. 15, n. 2, p. 119-125, 2011 . | pt_BR |
ISSN | 1678-4391 | |
URI | https://www.arca.fiocruz.br/handle/icict/7947 | |
Idioma | eng | pt_BR |
Editor | Elsevier | pt_BR |
Derechos de autor | open access | pt_BR |
Título | Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil. | pt_BR |
Tipo del documento | Article | pt_BR |
Resumen en Inglés | Amoebiasis is an infection caused by Entamoeba histolytica and is a potential health risk in countries in which health barriers are inappropriate. Since the discovery of Entamoeba dispar, the prevalence of amoebiasis has been modified. OBJECTIVE: This study has standardized the PCR technique applied for the diagnosis of different species of the E. histolytica/E. dispar complex and has evaluated the prevalence of infection among patients attending private and public clinical laboratories in Salvador City, Bahia State, Brazil. RESULTS: Analysis of 52,704 stool samples by microscopic examination demonstrated that 1,788 (3.4%) were positive for the E. histolytica/E. dispar complex and infection occurred more often in samples originated from public clinical laboratories (5.0%) than those that came from private laboratories (3.2%). PCR performed in approximately 15% (262) E. histolytica/E. dispar complex positive samples, randomly chosen, amplified 227 samples (86.6%), all of them positive for E. dispar. The non-amplified 35 samples (13.4%) were also negative for E. histolytica-specific galactose adhesin. Moreover, to exclude a probable infection caused by E. hartmanni, morphometric analysis demonstrated that non-amplified samples had cyst sizes comparable to E. histolytica/E. dispar (>10 µm). CONCLUSION: The absence of amplification of these samples indicates the presence of PCR inhibitors in the stool samples or the presence of DNA from Entamoeba species other than E. dispar, E. histolytica or E. hartmanni. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Health and Investigative Medicine Biotechnology . Salvador, BA, Brasil. Salvador, BA, Brasil | pt_BR |
Afiliación | Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratory of Pathology and Molecular Biology. Salvador, BA, Brasil | pt_BR |
Afiliación | Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil | pt_BR |
Palavras clave en Inglês | Amebiasis | pt_BR |
Palavras clave en Inglês | Entamoeba histolytica | pt_BR |
Palavras clave en Inglês | Diagnosis | pt_BR |
Palavras clave en Inglês | Prevalence | pt_BR |
DeCS | Entamoeba/genética | pt_BR |
DeCS | Entamebíase/diagnóstico | pt_BR |
DeCS | Entamebíase/parasitologia | pt_BR |
DeCS | Reação em Cadeia da Polimerase/métodos | pt_BR |
DeCS | Brasil/epidemiologia | pt_BR |
DeCS | DNA de Protozoário/análise | pt_BR |
DeCS | Diagnóstico Diferencial | pt_BR |
DeCS | Entamoeba/classificação | pt_BR |
DeCS | Entamoeba/isolamento & purificação | pt_BR |
DeCS | Entamoeba histolytica/genética | pt_BR |
DeCS | Entamoeba histolytica/isolamento & purificação | pt_BR |
DeCS | Entamebíase/epidemiologia | pt_BR |
DeCS | Fezes/parasitologia | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Prevalência | pt_BR |
DeCS | Sensibilidade e Especificidade | pt_BR |