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https://www.arca.fiocruz.br/handle/icict/8335
COMBINED OCCURRENCE OF TRYPANOSOMAL SIALIDASE/TRANS-SIALIDASE ACTIVITIES AND LEISHMANIAL METALLOPROTEINASE GENE HOMOLOGUES IN ENDOTRYPANUM SP.
Kinetoplastid
Metalloproteinase
Neuraminidase
Trans-sialidase
Sloth
Neuraminidase/genética
Trypanosomatina/enzimologia
Trypanosomatina/genética
Animais
Evolução Biológica
DNA de Protozoário/genética
Genes de Protozoários
Glicoproteínas/genética
Leishmania/enzimologia
Leishmania/genética
Hibridização de Ácido Nucleico
Especificidade da Espécie
Trypanosoma/enzimologia
Trypanosoma/genética
Trypanosomatina/classificação
Afiliação
The Rockfeller University. New York, NY, USA
The Rockfeller University. New York, NY, USA
New York University Medical Center. New York, NY, USA
New York University Medical Center. New York, NY, USA / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
The Rockfeller University. New York, NY, USA
New York University Medical Center. New York, NY, USA
New York University Medical Center. New York, NY, USA / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Resumo em Inglês
Endotrypanum (order Kinetoplastida: family Trypanosomatidae) is a parasite of forest dwelling tree sloths (Edentata: genera Choleopus and Bradypus). Unique among the haemoflagellates, this protozoan has an intraerythrocytic phase in the mammalian host. Nevertheless, many striking similarities exist between Endotrypanum and the human pathogen Leishmania that make it a useful model for epidemiological and evolutionary aspects of the biology of trypanosomatids. Importantly, Endotrypanum species share both the insect vector and host reservoir with certain species of Leishmania (subgenus Viannia). Because mixed infections with Endotrypanum and Leishmania are common in sloths and, therefore, likely to occur in the sandfly vector, there is a need for adequate biochemical markers to distinguish Endotrypanum from Leishmania infections. In this paper we show that Endotrypanum promastigotes possess sialidase and trans-sialidase activities, which are absent from Leishmania, and which are not closely related to the previously described trypanosomal sialidase/trans-sialidase enzyme. We also document the occurrence in Endotrypanum of homologues of the leishmanial surface metalloproteinase gp63 genes. The combined occurrence of sialidase/trans-sialidase activities and gp63 gene homologues in a unique organism has important ramifications for both field and laboratory studies on the biology of trypanosomatids, especially those related to host infection and evolution
Palavras-chave em inglês
EndototrypanumKinetoplastid
Metalloproteinase
Neuraminidase
Trans-sialidase
Sloth
DeCS
Metaloendopeptidases/genéticaNeuraminidase/genética
Trypanosomatina/enzimologia
Trypanosomatina/genética
Animais
Evolução Biológica
DNA de Protozoário/genética
Genes de Protozoários
Glicoproteínas/genética
Leishmania/enzimologia
Leishmania/genética
Hibridização de Ácido Nucleico
Especificidade da Espécie
Trypanosoma/enzimologia
Trypanosoma/genética
Trypanosomatina/classificação
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