Author | Acosta, Enrique Medina | |
Author | Pereira, Antonia Maria Ramos Franco | |
Author | Jansen, Ana Maria | |
Author | Sampol, Marcos | |
Author | Neves, Neuza Maria Alcântara | |
Author | Pontes-de-Carvalho, Lain Carlos | |
Author | Grimaldi Junior, Gabriel | |
Author | Nussenzweig, Victor | |
Access date | 2014-09-09T18:08:39Z | |
Available date | 2014-09-09T18:08:39Z | |
Document date | 1994 | |
Citation | MEDINA-ACOSTA, E. et al. Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids. European Journal Biochemical, v. 225, n. 1, p. 333-339, 1994. | pt_BR |
ISSN | 0014-2956 | |
URI | https://www.arca.fiocruz.br/handle/icict/8336 | |
Language | eng | pt_BR |
Publisher | FEBS | pt_BR |
Rights | open access | pt_BR |
Title | Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids. | pt_BR |
Type | Article | pt_BR |
Abstract | The expression of trans-sialidase and sialidase activities in the kinetoplastid protozoa was explored
as a potential marker to discriminate between the morphologically indistinguishable flagellates
isolated from human, insects and vertebrate reservoir hosts. By virtue of the differences observed
in the ratios of these enzyme activities, a collection of 52 species and strains comprising the
major taxa of these parasites could be separated into four expression types. Type-I parasites express
comparable levels of both trans-sialidase and sialidase activities (Endotrypanum species and Trypanosoma
lewisi). Type-I1 parasites express predominantly trans-sialidase activity (Trypanosoma cruzi
and Trypanosoma conorhini).Type-I11 parasites express sialidase activity exclusively (Trypanosoma
rangeli and Trypanosoma leeuwenhoeki). Type-IV parasites do not express either activity (Leishmania
species and Trypanoplasma borreli). The measurement of trans-sialidase and sialidase activities
thus permits the differentiation of parasites frequently found in the same insect vectors that are
difficult to distinguish, such as T. cruzi and 7: rangeli, or in the same sylvatic vertebrate and
invertebrate hosts, such as Leishmania and Endotrypanum. | pt_BR |
Affilliation | New York University Medical Center. Michael Heidelberger Division of Immunology. New York, USA | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | New York University Medical Center. Michael Heidelberger Division of Immunology. New York, USA / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | New York University Medical Center. Michael Heidelberger Division of Immunology. New York, USA / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | New York University Medical Center. Michael Heidelberger Division of Immunology. New York, USA / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | New York University Medical Center. Michael Heidelberger Division of Immunology. New York, USA | pt_BR |
DeCS | Neuraminidase/análise | pt_BR |
DeCS | Sialiltransferases/análise | pt_BR |
DeCS | Trypanosoma/classificação | pt_BR |
DeCS | Trypanosomatina/classificação | pt_BR |
DeCS | Animais | pt_BR |
DeCS | DNA de Protozoário/isolamento & purificação | pt_BR |
DeCS | Reservatórios de Doenças | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Insetos | pt_BR |
DeCS | Neuraminidase/biossíntese | pt_BR |
DeCS | Reação em Cadeia da Polimerase | pt_BR |
DeCS | Sialiltransferases/biossíntese | pt_BR |
DeCS | Trypanosoma/isolamento & purificação | pt_BR |
DeCS | Trypanosomatina/isolamento & purificação | pt_BR |
DeCS | Vertebrados | pt_BR |