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https://www.arca.fiocruz.br/handle/icict/8384
DISCOVERY OF MARKERS OF EXPOSURE SPECIFIC TO BITES OF LUTZOMYIA LONGIPALPIS, THE VECTOR OF LEISHMANIA INFANTUM CHAGASI IN LATIN AMERICA
Mordeduras e Picadas de Insetos/imunologia
Proteínas de Insetos/imunologia
Psychodidae/imunologia
Saliva/imunologia
Animais
Anticorpos/sangue
Cães
Raposas
Humanos
Proteínas de Insetos/uso diagnóstico
Proteínas de Insetos/genética
América Latina
Proteínas Recombinantes/uso diagnóstico
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Sensibilidade e Especificidade
Author
Teixeira, Clarissa Romero
Gomes, Regis Bernardo Brandim
Collin, Nicolas
Reynoso, David
Jochim, Ryan
Oliveira, Luiz Fabiano Borges
Seitz, Amy
Elnaiem, Dia-Eldin A
Caldas, Arlene de Jesus Mendes
Souza, Ana Paula Almeida de
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
Mendonça, Ivete Lopes de
Costa, Carlos Henrique Nery
Volf, Petr
Barral, Aldina Maria Prado
Kamhawi, Shaden
Valenzuela, Jesus G
Gomes, Regis Bernardo Brandim
Collin, Nicolas
Reynoso, David
Jochim, Ryan
Oliveira, Luiz Fabiano Borges
Seitz, Amy
Elnaiem, Dia-Eldin A
Caldas, Arlene de Jesus Mendes
Souza, Ana Paula Almeida de
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
Mendonça, Ivete Lopes de
Costa, Carlos Henrique Nery
Volf, Petr
Barral, Aldina Maria Prado
Kamhawi, Shaden
Valenzuela, Jesus G
Affilliation
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
University of Maryland Eastern Shore. Department of Natural Sciences. Princess Anne. Maryland, United States of America
Universidade Federal do Maranhão. São Luis, MA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil
Universidade Federal do Piaui. Instituto de Doenças Tropicais Natan Portella. Laboratório de Leishmanioses. Teresina, PI, Brasil
Universidade Federal do Piaui. Instituto de Doenças Tropicais Natan Portella. Laboratório de Leishmanioses. Teresina, PI, Brasil
Charles University in Prague. Faculty of Science. Department of Parasitology. Prague, Czech Republic
Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
University of Maryland Eastern Shore. Department of Natural Sciences. Princess Anne. Maryland, United States of America
Universidade Federal do Maranhão. São Luis, MA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil
Universidade Federal do Piaui. Instituto de Doenças Tropicais Natan Portella. Laboratório de Leishmanioses. Teresina, PI, Brasil
Universidade Federal do Piaui. Instituto de Doenças Tropicais Natan Portella. Laboratório de Leishmanioses. Teresina, PI, Brasil
Charles University in Prague. Faculty of Science. Department of Parasitology. Prague, Czech Republic
Fundação Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Salvador, BA, Brasil
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. Rockville, MD, United States of America
Abstract
BACKGROUND: Sand flies deliver Leishmania parasites to a host alongside salivary molecules that affect infection outcomes. Though some proteins are immunogenic and have potential as markers of vector exposure, their identity and vector specificity remain elusive. METHODOLOGY/PRINCIPAL FINDINGS: We screened human, dog, and fox sera from endemic areas of visceral leishmaniasis to identify potential markers of specific exposure to saliva of Lutzomyia longipalpis. Human and dog sera were further tested against additional sand fly species. Recombinant proteins of nine transcripts encoding secreted salivary molecules of Lu. longipalpis were produced, purified, and tested for antigenicity and specificity. Use of recombinant proteins corresponding to immunogenic molecules in Lu. longipalpis saliva identified LJM17 and LJM11 as potential markers of exposure. LJM17 was recognized by human, dog, and fox sera; LJM11 by humans and dogs. Notably, LJM17 and LJM11 were specifically recognized by humans exposed to Lu. longipalpis but not by individuals exposed to Lu. intermedia. CONCLUSIONS/SIGNIFICANCE: Salivary recombinant proteins are of value as markers of vector exposure. In humans, LJM17 and LJM11 emerged as potential markers of specific exposure to Lu. longipalpis, the vector of Leishmania infantum chagasi in Latin America. In dogs, LJM17, LJM11, LJL13, LJL23, and LJL143 emerged as potential markers of sand fly exposure. Testing these recombinant proteins in large scale studies will validate their usefulness as specific markers of Lu. longipalpis exposure in humans and of sand fly exposure in dogs.
DeCS
Vetores de DoençasMordeduras e Picadas de Insetos/imunologia
Proteínas de Insetos/imunologia
Psychodidae/imunologia
Saliva/imunologia
Animais
Anticorpos/sangue
Cães
Raposas
Humanos
Proteínas de Insetos/uso diagnóstico
Proteínas de Insetos/genética
América Latina
Proteínas Recombinantes/uso diagnóstico
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Sensibilidade e Especificidade
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