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https://www.arca.fiocruz.br/handle/icict/9423
VIRULENT AND AVIRULENT STRAINS OF TOXOPLASMA GONDII WHICH DIFFER IN THEIR GLYCOSYLPHOSPHATIDYLINOSITOL CONTENT INDUCE SIMILAR BIOLOGICAL FUNCTIONS IN MACROPHAGES
Cercopithecus aethiops
Glycosylphosphatidylinositols/metabolism
Toxoplasma/pathogenicity
Tumor Necrosis Factor-alpha/metabolism
Author
Affilliation
Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany /Institute of Molecular and Cellular Biology. Strasbourg, France.
University of St Andrews. Biomedical Sciences Research Complex. St Andrews, United Kingdom.
Philipps University. Institute for Virology.Laboratory of Parasitology. Marburg, Germany.
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil.
University of Montpellier. Montpellier, France.
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Departamento de Bioquimica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG,Brazil / University of Massachusetts Medical School. Department of Medicine. Division of Infectious Diseases and Immunology. Worcester, MA, USA.
Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany/Lille 1 University. Unit of Structural and Functional Glycobiology. Lille, France.
Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany/ University of Tours. Infectiology and Public Health. Tours, France.
University of St Andrews. Biomedical Sciences Research Complex. St Andrews, United Kingdom.
Philipps University. Institute for Virology.Laboratory of Parasitology. Marburg, Germany.
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil.
University of Montpellier. Montpellier, France.
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Departamento de Bioquimica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG,Brazil / University of Massachusetts Medical School. Department of Medicine. Division of Infectious Diseases and Immunology. Worcester, MA, USA.
Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany/Lille 1 University. Unit of Structural and Functional Glycobiology. Lille, France.
Philipps University. Institute for Virology. Laboratory of Parasitology. Marburg, Germany/ University of Tours. Infectiology and Public Health. Tours, France.
Abstract
Glycosylphosphatidylinositols (GPIs) from several protozoan parasites are thought to elicit a detrimental stimulation of the host innate immune system aside their main function to anchor surface proteins. Here we analyzed the GPI biosynthesis of an avirulent Toxoplasma gondii type 2 strain (PTG) by metabolic radioactive labeling. We determined the biological function of individual GPI species in the PTG strain in comparison with previously characterized GPI-anchors of a virulent strain (RH). The GPI intermediates of both strains were structurally similar, however the abundance of two of six GPI intermediates was significantly reduced in the PTG strain. The side-by-side comparison of GPI-anchor content revealed that the PTG strain had only ∼ 34% of the protein-free GPIs as well as ∼ 70% of the GPI-anchored proteins with significantly lower rates of protein N-glycosylation compared to the RH strain. All mature GPIs from both strains induced comparable secretion levels of TNF-α and IL-12p40, and initiated TLR4/MyD88-dependent NF-κBp65 activation in macrophages. Taken together, these results demonstrate that PTG and RH strains differ in their GPI biosynthesis and possess significantly different GPI-anchor content, while individual GPI species of both strains induce similar biological functions in macrophages.
Keywords
Toxoplasma gondiiCercopithecus aethiops
Glycosylphosphatidylinositols/metabolism
Toxoplasma/pathogenicity
Tumor Necrosis Factor-alpha/metabolism
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