Author | Furtado, Yara | |
Author | Almeida, Gutemberg | |
Author | Silveira, Filomena Aste | |
Author | Silva, Kátia S. | |
Author | Maldonado, Paula | |
Author | Val, Isabel Cristina do | |
Author | Cavalcanti, Silvia | |
Author | Miranda-Alves, L. | |
Author | Carvalho, Maria da Gloria da Costa | |
Access date | 2015-03-19T13:30:18Z | |
Available date | 2015-03-19T13:30:18Z | |
Document date | 2015 | |
Citation | FURTADO, Yara et al. TIMP-2 gene methylation in cervical precursor and invasive lesions. Experimental and Molecular Pathology, New York, v. 98, p. 119–123, Feb. 2015. | pt_BR |
ISSN | 0014-4800 | |
URI | https://www.arca.fiocruz.br/handle/icict/9742 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Title | TIMP-2 gene methylation in cervical precursor and invasive lesions | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.yexmp.2015.01.008 | |
Abstract | Objective:To analyze the presence of HPV-DNA and TIMP-2 gene methylation in cervical precursor and invasive lesions, aswell as to study the associations among the latter, the presence of HPV-DNA, and the clinical evolution of such lesions. Methods:Cross-sectional study that includes 49 biopsy or brush smear samples from women with a normal cer-vix, LSIL, HSIL, microinvasive carcinoma and invasive carcinoma. The presence of HPV-DNA and specificmethyl-ation was analyzed using PCR. Thirty-eight biopsy samples for HSIL, microinvasive carcinoma and frank invasive carcinoma as well as 11 brush smear samples for LSIL and normal cervices were analyzed. Results:TIMP-2 gene methylation was detected in 86.8% (33/38) of the samples fromthe group with lesions and 50% (4/8) of the normal samples (p= 0.03). HPV-DNA was detected in 81.6% (31/38) of the samples from the group with lesions and 25% (2/8) of the normal samples (p= 0.003). HPV-DNA wasmore frequent in the meth-ylated samples (50%), and the group with methylation had a higher risk of unfavorable evolution than the group without methylation; however, such observations were not statistically significant (p=0.19). Conclusion:TIMP-2genemethylationand thepresenceofHPV-DNAwere characteristic of thegroupwithcervical lesions. Methylation was not associated with the presence of HPV-DNA or an unfavorable clinical evolution. | pt_BR |
Affilliation | Universidade Federal do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Programa de Pós-Graduação em Ciências Cirúrgicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Ginecologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Programa de Pós-Graduação em Ciências Cirúrgicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Ginecologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Programa de Pós-Graduação em Ciências Cirúrgicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Ginecologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Mulheres, Crianças e Adolescentes Fernandes Figueira. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ginecologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Patologia. Laboratório de Patologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Methylation | pt_BR |
Subject | TIMP-2 | pt_BR |
Subject | Papillomavirus Infection | pt_BR |
Subject | Cervix | pt_BR |
Subject | Neoplasia | pt_BR |
DeCS | Metilação | pt_BR |
DeCS | Inibidor Tecidual de Metaloproteinase-2 | pt_BR |
DeCS | Infecções por Papillomavirus | pt_BR |
DeCS | Neoplasias | pt_BR |
Embargo date | 2030-12-31 | |