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ENDOGENOUS INTERLEUKIN-4 DOWNREGULATES THE TYPE 1 CD4 T CELL-MEDIATED IMMUNE RESPONSE INDUCED BY INTRAMUSCULAR DNA IMMUNIZATION.
Interleucina-4/imunologia
Vacinas de DNA/farmacologia
Animais
Linfócitos T CD4-Positivos/efeitos de drogas
Imunidade Celular/efeitos de drogas
Imunoglobulina G/sangue
Injeções Intramusculares
Interleucina-4/deficiência
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Baço/imunologia
Vacinas de DNA/administração & dosagem
Autor
Afiliación
Universidade Federal de São Paulo. Escola Paulista de Medicina. 1Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de São Paulo. Escola Paulista de Medicina. 1Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de São Paulo. Escola Paulista de Medicina. 1Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Resumen en ingles
Intramuscular (i.m.) administration of eukaryotic plasmid vectors containing foreign genes is a general immunization
strategy capable of inducing protective type 1 immune responses against viral, bacterial, fungal,
and parasitic infections. We have described that immunization with a plasmid containing a gene encoding a
parasite antigen elicits specific type 1 protective immune responses against experimental infection with the
human protozoan parasite Trypanosoma cruzi. However, we had evidence suggesting that DNA immunization
concomitantly activated specific type 2 immune responses. To determine precisely the influence of the type 2
cytokine interleukin-4 (IL-4) during DNA immunization, we compared the immune responses of genetically
modified IL-4-deficient or wild-type (wt) BALB/c mice. IL-4-deficient mice had a significantly lower ratio of
specific serum IgG1/IgG2a, and on in vitro restimulation with antigen, their spleen cells secreted significantly
higher amounts of interferon-g (IFN-g). In contrast, absence of IL-4 did not affect total serum antibody response,
T cell proliferative responses, or activation of IFN-g-producing CD81 T cells. Our results suggested
that in contrast to conventional adjuvants, such as alum and complete Freund’s adjuvant, specific IgG1 in
DNA-immunized BALB/c mice was highly dependent on IL-4. To our knowledge, our study provides the first
evidence that endogenous IL-4 selectively downregulates the type 1 CD41 T cell-mediated immune response
induced by i.m. genetic immunization, a fact that may have implications for the design of certain DNA vaccines.
DeCS
Linfócitos T CD4-Positivos/imunologiaInterleucina-4/imunologia
Vacinas de DNA/farmacologia
Animais
Linfócitos T CD4-Positivos/efeitos de drogas
Imunidade Celular/efeitos de drogas
Imunoglobulina G/sangue
Injeções Intramusculares
Interleucina-4/deficiência
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Baço/imunologia
Vacinas de DNA/administração & dosagem
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