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ArtigoDireito Autoral
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- IOC - Artigos de Periódicos [12500]
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MICROCYSTIN ACCUMULATION IN CLADOCERANS: FIRST EVIDENCE OF MC UPTAKE FROM AQUEOUS EXTRACTS OF A NATURAL BLOOM SAMPLE
10.1016/j.toxicon.2014.05.015
Afiliação
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil / Universidad de Antioquia. Facultad de Ciencias Exactas y Naturales. Grupo de Quimica Orgánica de Productos Naturales (QOPN), Medellin, Colombia.
Universidad de Antioquia. Facultad de Ciencias Exactas y Naturales. Grupo de Quimica Orgánica de Productos Naturales (QOPN), Medellin, Colombia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil / Universidad de Antioquia. Facultad de Ciencias Exactas y Naturales. Grupo de Quimica Orgánica de Productos Naturales (QOPN), Medellin, Colombia.
Universidad de Antioquia. Facultad de Ciencias Exactas y Naturales. Grupo de Quimica Orgánica de Productos Naturales (QOPN), Medellin, Colombia.
Resumo em Inglês
Bioaccumulation of microcystins (MC) in zooplankton has been shown in several studies,
mainly in field samples. A few studies, however, have demonstrated MC bioaccumulation
in laboratory experiments. Although ingestion of cell-bound MC is considered the main
route of MC accumulation, another important source is the MC from the dissolved fraction
(DMC). This study reports the accumulation of DMC from aqueous extracts of natural
bloom samples in three cladoceran species: Moina micrura, Daphnia laevis and Daphnia
similis. Animals were exposed for 96 h to aqueous extracts of lyophilized matter from two
bloom samples from Colombian reservoirs in different concentrations (25
e1000 mg DW L 1). Analysis by HPLC-MS detected MC-LR in these samples at concentrations
of 434e538 mg g 1. For the analysis of MC in animal tissues the samples were
homogenized and sonicated in methanol:water (75%) and analyzed by ELISA. Results
showed that the animals uptake of MC increased with increasing exposure concentrations
of aqueous extracts, with M. micrura and D. laevis clones presenting the highest MC concentrations
in their tissues (up to 1170e1260 mg g 1) while D. similis the lowest
(184 mg g 1). This study shows, for the first time, that MC uptake from dissolved fraction by
zooplankton is possible, not only from the ingestion of seston or cell-bound MC as previously
demonstrated.
Editor
Elsevier
Referência
FERRÃO-FILHO, A.S.; HERRERA, N.; ECHEVERRI, L.F. Microcystin accumulation in cladocerans: First evidence of MC uptake from aqueous extracts of a natural bloom sample. Toxicon, n.87, p.26-31, 2014.ISSN
0041-010110.1016/j.toxicon.2014.05.015
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