The use of electromagnetic fields has been reported to enhance peripheralnerve regeneration. This study aimed to identify the effects of a prolonged protocolof low-frequency pulsed electromagnetic field (PEMF) on peripheral nerve regeneration.Thirty-four male Swiss mice (Mus musculus) were divided into PEMF (n= 17) and control(n= 17) groups. All animals underwent a unilateral sciatic-crush lesion, and the PEMF groupwas exposed to a 72-Hz, 2-G electromagnetic field for 30 min, five days a week, for threeweeks. Functional analysis was carried out weekly. After three weeks, the animals wereeuthanized, and histological, morphometric, oxidative stress, and TGF-β1 analyses wereperformed. Functional analysis showed no differences between the groups. Histologicalappearance was similar between PEMF and control nerves. Morphometric assessmentshowed that the PEMF nerves trended toward decreased regeneration. The levels of freeradicals were more pronounced in PEMF nerves, but were not associated with an increasein the content of the TGF-β1/Smad signaling pathway. Prolonged PEMF regimen leads todelayed histological peripheral nerve regeneration and increased oxidative stress but noloss of function recovery