| Author | Silva-Gomes, Natália Lins | |
| Author | Ennes-Vidal, Vitor | |
| Author | Carolo, Julliane Castro Ferreira | |
| Author | Batista, Marcos Meuser | |
| Author | Soeiro, Maria de Nazaré Correia | |
| Author | Menna-Barreto, Rubem | |
| Author | Moreira, Otacílio Cruz | |
| Access date | 2015-04-17T17:04:34Z | |
| Available date | 2015-04-17T17:04:34Z | |
| Document date | 2014 | |
| Citation | SILVA-GOMES, Natália Lins et al. Nucleoside triphosphate diphosphohydrolase1 (TcNTPDase-1) gene expression is increased due to heat shock and in infective forms of Trypanosoma cruzi. Parasites & Vectors, n.7:243, 6p, 2014. | pt_BR |
| ISSN | 1756-3305 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10077 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Patogenicidade | pt_BR |
| Title | Nucleoside triphosphate diphosphohydrolase1 (TcNTPDase-1) gene expression is increased due to heat shock and in infective forms of Trypanosoma cruzi | pt_BR |
| Type | Article | |
| DOI | 10.1186/s13071-014-0463-0 | pt_BR |
| Abstract | Background: Ecto-Nucleoside Triphosphate Diphosphohydrolases (Ecto-NTPDases) are enzymes that hydrolyze tri- and/or
di-phosphate nucleotides. Evidences point to their participation in Trypanosoma cruzi virulence and infectivity. In this work,
we evaluate TcNTPDase-1 gene expression in comparison with ecto-NTPDase activity, in order to study the role of
TcNTPDase-1 in parasite virulence, infectivity and adaptation to heat shock.
Findings: Comparison between distinct T. cruzi isolates (Y, 3663 and 4167 strains, and Dm28c, LL014 and CL-14 clones)
showed that TcNTPDase-1 expression was 7.2 ± 1.5 times higher in the Dm28c than the CL-14 avirulent clone. A remarkable
expression increase was also observed in the trypomastigote and amastigote forms (22.5 ± 5.6 and 16.3 ± 3.8 times higher
than epimastigotes, respectively), indicating that TcNTPDase-1 is overexpressed in T. cruzi infective forms. Moreover, heat
shock and long-term cultivation also induced a significant increment on TcNTPDase-1 expression.
Conclusions: Our results suggest that TcNTPDase-1 plays an important role on T. cruzi infectivity and adaptation to stress
conditions, such as long-term cultivation and heat shock. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratorio de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Gene expression | pt_BR |
| Subject | Infectivity | pt_BR |
| Subject | Virulence | pt_BR |
| DeCS | Expressão Gênica | pt_BR |
| DeCS | Virulência | pt_BR |
