Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10435
Title: Early transplantation of bone marrow mononuclear cells promotes neuroprotection and modulation of inflammation after status epilepticus in mice by paracrine mechanisms.
Authors: Leal, Marcos Maurício Tosta
Ferro, Zaquer Suzana Munhoz Costa
Souza, Bruno Solano de Freitas
Azevedo, Carine Machado
Carvalho, Thiago Meneses
Kaneto, Carla Martins
Carvalho, Rejane Hughes
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Affilliation: Hospital São Rafael. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Hospital São Rafael. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Status epilepticus (SE) is a severe clinical manifestation of epilepsy associated with intense neuronal loss and inflammation, two key factors involved in the pathophysiology of temporal lobe epilepsy. Bone marrow mononuclear cells (BMMC) attenuated the consequences of pilocarpine-induced SE, including neuronal loss, in addition to frequency and duration of seizures. Here we investigated the effects of BMMC transplanted early after the onset of SE in mice, as well as the involvement of soluble factors produced by BMMC in the effects of the cell therapy. Mice were injected with pilocarpine for SE induction and randomized into three groups: transplanted intravenously with 1 × 10(7) BMMC isolated from GFP transgenic mice, injected with BMMC lysate, and saline-treated controls. Cell tracking, neuronal counting in hippocampal subfields and cytokine analysis in the serum and brain were performed. BMMC were found in the brain 4 h following transplantation and their numbers progressively decreased until 24 h following transplantation. A reduction in hippocampal neuronal loss after SE was found in mice treated with live BMMC and BMMC lysate when compared to saline-treated, SE-induced mice. Moreover, the expression of inflammatory cytokines IL-1ß, TNF-α, IL-6 was decreased after injection of live BMMC and to a lesser extent, of BMMC lysate, when compared to SE-induced controls. In contrast, IL-10 expression was increased. Analysis of markers for microglia activation demonstrated a reduction of the expression of genes related to type 1-activation. BMMC transplantation promotes neuroprotection and mediates anti-inflammatory effects following SE in mice, possibly through the secretion of soluble factors.
DeCS: Transplante de Medula Óssea
Fármacos Neuroprotetores
Pilocarpina/administração & dosagem
Estado Epiléptico/induzido quimicamente
Animais
Sequência de Bases
Citocinas/biossíntese
Primers do DNA
Expressão Gênica
Proteínas de Fluorescência Verde/genética
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Microglia/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Estado Epiléptico/cirurgia
Issue Date: 2014
Publisher: Springer Verlag
Citation: LEAL, M. M. T. et al. Early transplantation of bone marrow mononuclear cells promotes neuroprotection and modulation of inflammation after status epilepticus in mice by paracrine mechanisms. Neurochemical Research, v. 39, n. 2, p. 259-268, 2014.
ISSN: 1573-6903
10.1007/s11064-013-1217-7
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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