| Author | Mota, Laura Azeredo Miranda | |
| Author | Roberto Neto, João | |
| Author | Monteiro, Verônica Gomes | |
| Author | Lobato, Caroliny Samary Silva | |
| Author | Oliveira, Marco Antonio de | |
| Author | Cunha, Maura da | |
| Author | D'Avila, Heloisa | |
| Author | Seabra, Sérgio Henrique | |
| Author | Bozza, Patricia Torres | |
| Author | DaMatta, Renato Augusto | |
| Access date | 2015-05-27T13:39:52Z | |
| Available date | 2015-05-27T13:39:52Z | |
| Document date | 2014 | pt_BR |
| Citation | MOTA, Laura Azeredo et al. Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii. Mem Inst Oswaldo Cruz, v.109, n.6, p.767-774, Sept. 2014. | pt_BR |
| ISSN | 1678-8060 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/10554 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz | pt_BR |
| Rights | open access | pt_BR |
| Title | Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/0074-0276140119 | pt_BR |
| Abstract | Lipid bodies [lipid droplets (LBs)] are lipid-rich organelles involved in lipid metabolism, signalling and inflammation. Recent findings suggest a role for LBs in host response to infection; however, the potential functions of this organelle in Toxoplasma gondii infection and how it alters macrophage microbicidal capacity during infection are not well understood. Here, we investigated the role of host LBs in T. gondii infection in mouse peritoneal macrophages in vitro. Macrophages cultured with mouse serum (MS) had higher numbers of LBs than those cultured in foetal bovine serum and can function as a model to study the role of LBs during intracellular pathogen infection. LBs were found in association with the parasitophorous vacuole, suggesting that T. gondii may benefit from this lipid source. Moreover, increased numbers of macrophage LBs correlated with high prostaglandin E2 (PGE2) production and decreased nitric oxide (NO) synthesis. Accordingly, LB-enriched macrophages cultured with MS were less efficient at controlling T. gondii growth. Treatment of macrophages cultured with MS with indomethacin, an inhibitor of PGE2 production, increased the microbicidal capacity against T. gondii. Collectively, these results suggest that culture with MS caused a decrease in microbicidal activity of macrophages against T. gondii by increasing PGE2 while lowering NO production. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil / Universidade do Vale do Paraíba. Instituto de Pesquisa e Desenvolvimento. Laboratório de Parasitologia e Biotecnologia. São José dos Campos, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal de Juiz de Fora. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Centro Universitário Estadual da Zona Oeste. Laboratório de Tecnologia em Bioquímica e Microscopia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil. | pt_BR |
| Subject | Macrophages | pt_BR |
| Subject | Lipid bodies | pt_BR |
| Subject | Nitric oxide | pt_BR |
| Subject | Óxido Nítrico | pt_BR |
| Subject | Microbicidal capacity | pt_BR |
| Subject | Toxoplasma gondii | pt_BR |
| DeCS | Toxoplasma | pt_BR |
| DeCS | Macrófagos | pt_BR |
| DeCS | Óxido Nítrico | pt_BR |
| DeCS | Prostaglandinas | pt_BR |
