Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10586
Title: Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil
Authors: Pereira Júnior, Antonio Marques
Teles, Carolina Bioni Garcia
Santos, Ana Paula de Azevedo dos
Rodrigues, Moreno de Souza
Marialva, Eric Fabrício
Pessoa, Felipe Arley Costa
Medeiros, Jansen Fernandes
Affilliation: Fundação Oswaldo Cruz. Instituto Nacional de Pesquisas da Amazônia. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis da Amazônia. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis da Amazônia. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis da Amazônia. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis da Amazônia.. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis da Amazônia. EDTA. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Laboratório de Entomologia. Porto Velho, RO, Brasil.
Abstract: Background: Phlebotomine sand flies (Diptera: Psychodidae) are insects of medical importance due to the role that some species play in the transmission of leishmaniasis. This work aimed to study some ecological aspects among sand flies fauna inhabiting two different environments: the várzea (lowland Amazonian forest) and terra firme (upland Amazonian forest), both located in Tefé Municipality, Amazonas State, Braziland to detect Leishmania infection in those phlebotomine populations. Methods: Sand flies were collected using HP light traps. Collection took place over the course of six months: January, February, April, August, September, and October of 2013. To detect natural infection by Leishmania, DNA samples were extracted from female sand flies and submitted to Polymerase Chain Reaction (PCR) targeting the kDNA gene; Leishmania species were identified by PCR-RFLP targeting the hsp70 gene and genetic sequencing. Results: In all, 5,716 individuals were collected, and 46 species were identified. Trichophoromyia ubiquitalis (3,330 – 58.26%) and Nyssomyia antunesi (661 – 11.26%) were the most abundant species. Species richness was greater in terra firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species) were richer than peridomiciles (28 species). DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality.Conclusions: The high abundance of Th. ubiquitalis and Ps. davisi and detection of DNA of Leishmania sp. may indicate that both species could be putative vectors for American Cutaneous Leishmaniasis (ACL) in the terra firme environment of Tefé. The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric stress during part of the year that could influence the local diversity, if compared with other studies. This is the first report in Amazonas State of Th. ubiquitalis with presence of L. (V.) lainsoni DNA
Keywords: Diversity
Amazon environments
Cutaneous Leishmaniasis
Richness
Diversity
Vectors
Issue Date: 2015
Publisher: BioMed Central
Citation: PEREIRA JÚNIOR, A. M. et al. Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil. Parasites & Vectors, v. 8, p.180, 2015.
DOI: 10.1186/s13071-015-0789-2
ISSN: 1756-3305
Copyright: open access
Appears in Collections:AM - ILMD - Artigos de Periódicos
RO - Artigos de Periódicos
BA - IGM - Artigos de Periódicos

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