Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10674
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dc.contributor.authorVieira, Cecilia Stahl
dc.contributor.authorAlmeida, Diogo Burigo
dc.contributor.authorThomaz, André Alexandre de
dc.contributor.authorMenna-Barreto, Rubem Figueredo Sadok
dc.contributor.authorSantos-Mallet, Jacenir R.
dc.contributor.authorCesar, Carlos Lenz
dc.contributor.authorGomes, Suzete Araujo Oliveira
dc.contributor.authorFeder, Denise
dc.date.accessioned2015-06-08T14:01:45Z
dc.date.available2015-06-08T14:01:45Z
dc.date.issued2011
dc.identifier.citationVIEIRA, Cecilia Stahl et al. Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi. Mem Inst Oswaldo Cruz, v.106, n.2, p.158-165, Mar. 2011.
dc.identifier.issn1678-8060
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/10674
dc.language.isopor
dc.publisherFundação Oswaldo Cruz
dc.rightsopen access
dc.titleStudying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
dc.typeArticle
dc.description.abstractenSemiconductor nanoparticles, such as quantum dots (QDs), were used to carry out experiments in vivo and ex vivo with Trypanosoma cruzi. However, questions have been raised regarding the nanotoxicity of QDs in living cells, microorganisms, tissues and whole animals. The objective of this paper was to conduct a QD nanotoxicity study on living T. cruzi protozoa using analytical methods. This was accomplished using in vitro experiments to test the interference of the QDs on parasite development, morphology and viability. Our results show that after 72 h, a 200 μM cadmium telluride (CdTe) QD solution induced important morphological alterations in T. cruzi, such as DNA damage, plasma membrane blebbing and mitochondrial swelling. Flow cytometry assays showed no damage to the plasma membrane when incubated with 200 μM CdTe QDs for up to 72 h (propidium iodide cells), giving no evidence of classical necrosis. Parasites incubated with 2 μM CdTe QDs still proliferated after seven days. In summary, a low concentration of CdTe QDs (2 μM) is optimal for bioimaging, whereas a high concentration (200 μM CdTe) could be toxic to cells. Taken together, our data indicate that 2 μM QD can be used for the successful long-term study of the parasite-vector interaction in real time.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade Estadual de Campinas.Instituto de Física Gleb Wataghin. Laboratório de Aplicações Biomédicas de Lasers. Campinas, SP, Brasil.
dc.creator.affilliationUniversidade Estadual de Campinas.Instituto de Física Gleb Wataghin. Laboratório de Aplicações Biomédicas de Lasers. Campinas, SP, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade Estadual de Campinas.Instituto de Física Gleb Wataghin. Laboratório de Aplicações Biomédicas de Lasers. Campinas, SP, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil
dc.creator.affilliationUniversidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil
dc.subject.enCdTe quantum dots
dc.subject.enTrypanosoma cruzi
dc.subject.enNanotoxicity
dc.subject.decsTrypanosoma cruzi
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