Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10699
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dc.contributor.authorCastro Junior, José Geraldo
dc.contributor.authorFreire, Mariana Lourenço
dc.contributor.authorCampos, Samantha Priscila Silva
dc.contributor.authorScopel, Kezia K. G
dc.contributor.authorPorrozzi, Renato
dc.contributor.authorSilva, Edmilson Domingos da
dc.contributor.authorColombo, Fábio A
dc.contributor.authorSilveira, Rita de Cássia Viveiros da
dc.contributor.authorMarques, Marcos José
dc.contributor.authorCoimbra, Elaine Soares
dc.date.accessioned2015-06-08T14:01:52Z
dc.date.available2015-06-08T14:01:52Z
dc.date.issued2014
dc.identifier.citationCASTRO JUNIOR, José Geraldo et al. Evidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays. Rev. Inst. Med. Trop. Sao Paulo, v.56, n.3, p.225-229, May-June, 2014.
dc.identifier.issn0036-4665
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/10699
dc.language.isoeng
dc.publisherInstituto de Medicina Tropical de São Paulo
dc.rightsopen access
dc.titleEvidence of Leishmania (Leishmania) infantum infection in dogs from Juiz de Fora, Minas Gerais State, Brazil, based on immunochromatographic Dual-Path Platform (DPP) and PCR assays
dc.typeArticle
dc.identifier.doi10.1590/S0036-46652014000300008
dc.description.abstractenIn Brazil, domestic dogs are branded as the primary reservoir for zoonotic visceral leishmaniasis, due to the clear positive correlation observed between human and canine infection rates. This study aimed to carry out a serological survey of canine visceral leishmaniasis (CVL) in dogs housed at a public kennel in the municipality of Juiz de Fora, Minas Gerais State, Brazil, using the immunochromatographic TR DPP® CVL rapid test. Additionally, conventional and/or real time PCR assay was used to detect and confirm L. infantum infection in the DPP positive dogs only. Of the 400 dogs studied, most did not present clinical signs for CVL (p < 0.05), and fifteen (3.8%) were seropositive in the DPP test. There was no statistically significant difference between the DPP seropositive dogs and the clinical signs of the disease (p > 0.05). Both conventional and real time PCR tests confirmed L. infantum infection in nine (75.0%) of the twelve DPP seropositive dogs that remained alive during the follow-up period. This study is the first seroepidemiologic survey of CVL held in the city of Juiz de Fora, and the results reinforce the idea that this disease is currently in a process of expansion and urbanization in Brazil. Furthermore, this study highlights the use of the DPP test as an alternative for diagnosing CVL in large and mid-sized cities, due to its ease of implementation.
dc.creator.affilliationUniversidade Federal de Juiz de Fora. Programa de Pós-graduação em Saúde Brasileira. Juiz de Fora, MG, Brasil / Secretaria Municipal de Saúde de Juiz de Fora. Departamento de Vigilância Epidemiológica e Ambiental. Setor de Zoonoses. Juiz de Fora, MG, Brasil.
dc.creator.affilliationUniversidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.
dc.creator.affilliationUniversidade Federal de Juiz de Fora. Intituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.
dc.creator.affilliationUniversidade Federal de Juiz de Fora. Intituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Biomanguinhos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade Federal de Alfenas. Instituto de Ciências Biomédicas. Departamento de Patologia e Parasitologia. Laboratório de Parasitologia. Alfenas, MG, Brasil.
dc.creator.affilliationUniversidade Federal de Alfenas. Instituto de Ciências Biomédicas. Departamento de Patologia e Parasitologia. Laboratório de Parasitologia. Alfenas, MG, Brasil.
dc.creator.affilliationUniversidade Federal de Alfenas. Instituto de Ciências Biomédicas. Departamento de Patologia e Parasitologia. Laboratório de Parasitologia. Alfenas, MG, Brasil.
dc.creator.affilliationUniversidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.
dc.subject.enCanine visceral leishmaniasis
dc.subject.enSerological diagnosis
dc.subject.enImmunochromatographic diagnosis
dc.subject.enDPP
dc.subject.enPCR diagnosis
dc.subject.decsLeishmaniose Visceral
dc.subject.decsCães
dc.subject.decsDiagnóstico
dc.subject.decsReação em Cadeia da Polimerase
Appears in Collections:Biomanguinhos - Artigos de Periódicos
IOC - Artigos de Periódicos

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