Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10704
Title: Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
Authors: Bonin, Renata Fajardo
Chapeaurouge, Alex
Perales, Jonas
Silva Jr., José Godinho da
Nascimento, Hilton Jorge do
Assef, Ana Paula D`Alincourt Carvalho
Senna, José Procópio Moreno
Affilliation: Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.
undação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.
undação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa de Infecções Hospitalares. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.
Abstract: Purpose: Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised patients. This pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly problematic because of its prevalence and resistance patterns to several antibiotics. In the present study, we used an immunoproteome-based approach to identify immunogenic proteins located on the surface of A. baumannii for the development of a possible immunotherapy against this devastating bacterial infection. Experimental design: Sera from patients with A. baumannii infections (n = 50) and from a control group of healthy individuals (n = 3) were analyzed for reactivity against A. baumannii outer membrane proteins (OMPs) using Western blot analysis. To identify potential immunogenic proteins in A. baumannii,OMPs were separated by 2DE (2Delectrophoresis), and reactive sera from infected patients were randomly selected and divided into two different pools, each containing 15 sera. Finally, MALDI-TOF/TOF mass spectrometric analysis was employed to identify the corresponding proteins. Results: This analysis identified six immunoreactive proteins: OmpA, Omp34kDa, OprC, OprB-like, OXA-23, and ferric siderophore receptor protein. Notably, these proteins are highly abundant on the bacterial surface and involved in virulence, antibiotic resistance, and growth. Conclusions and clinical relevance: Our results support the notion that the proteins identified in the present immunoproteome study could serve as antigen candidates for the development of vaccines and passive immunotherapies against A. baumannii infections.
Keywords: Acinetobacter baumannii
Hospital infection
Immunoproteomics
MALDI-TOF/TOF
keywords: Infecção hospitalar
DeCS: Acinetobacter baumannii
Proteômica
Issue Date: 2014
Publisher: WILEY-VCH Verlag GmbH & Co
Citation: BONIN, Renata Fajardo et al. Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach. Proteomics Clin. Appl., v.00, p.1-8, 2014.
DOI: 10.1002/prca.201300133
ISSN: 1862-8354
Copyright: open access
Appears in Collections:Biomanguinhos - Artigos de Periódicos
IOC - Artigos de Periódicos

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