Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10869
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dc.contributor.authorMoreno, Luisa Zanolli
dc.contributor.authorPaixão, Renata
dc.contributor.authorGobbi, Debora Dirani Sena de
dc.contributor.authorRaimundo, Daniele Cristine
dc.contributor.authorFerreira, Thais Sebastiana Porfida
dc.contributor.authorMoreno, Andrea Micke
dc.contributor.authorHofer, Ernesto
dc.contributor.authorReis, Cristhiane Moura Falavina dos
dc.contributor.authorMatté, Glavur Rogério
dc.contributor.authorMatté, Maria Helena
dc.date.accessioned2015-06-17T12:05:21Z
dc.date.available2015-06-17T12:05:21Z
dc.date.issued2014
dc.identifier.citationMORENO, Luisa Zanolli et al. Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets. BioMed Research International, v.20, Article ID 742032, 12p, 2014.
dc.identifier.issn2314-6133
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/10869
dc.language.isoeng
dc.publisherHindawi Pùblishing Corporation
dc.rightsopen access
dc.titlePhenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets
dc.typeArticle
dc.identifier.doi10.1155/2014/742032
dc.description.abstractenIn the last decade, atypical Listeria monocytogenes and L. innocua strains have been detected in food and the environment. Because of mutations in the major virulence genes, these strains have different virulence intensities in eukaryotic cells. In this study, we performed phenotypic and genotypic characterization of atypical L. monocytogenes and L. innocua isolates obtained from swine slaughterhouses and meat markets. Forty strains were studied, including isolates of L. monocytogenes and L. innocua with low-hemolytic activity. The isolates were characterized using conventional phenotypic Listeria identification tests and by the detection and analysis of L. monocytogenes-specific genes. Analysis of 16S rRNA was used for the molecular identification of the Listeria species. The L. monocytogenes isolates were positive for all of the virulence genes studied. The atypical L. innocua strains were positive for hly, plcA, and inlC. Mutations in the InlC, InlB, InlA, PI-PLC, PC-PLC, and PrfA proteins were detected in the atypical isolates. Further in vitro and transcriptomic studies are being developed to confirm the role of these mutations in Listeria virulence.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Saúde Pública. Laboratório Prática de Saúde Pública. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Saúde Pública. Laboratório Prática de Saúde Pública. São Paulo, SP, Brasil.
dc.creator.affilliationUniversidade de São Paulo. Faculdade de Saúde Pública. Laboratório Prática de Saúde Pública. São Paulo, SP, Brasil.
dc.subject.enListeria monocytogenes
dc.subject.enListeria innocua
dc.subject.decsListeria
dc.subject.decsSuínos
dc.subject.decsVirulência
dc.subject.decsDoenças dos Suínos
dc.subject.decsMatadouros
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