Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/10872
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dc.contributor.authorMenezes, Francisca Gleire Rodrigues de
dc.contributor.authorNeves, Soraya da Silva
dc.contributor.authorSousa, Oscarina Viana de
dc.contributor.authorVila-Nova, Candida Machado Veira Maia
dc.contributor.authorMaggioni, Rodrigo
dc.contributor.authorTheophilo, Grace Nazareth Diogo
dc.contributor.authorHofer, Ernesto
dc.contributor.authorVieira, Regine Helena Silva dos Fernandes
dc.date.accessioned2015-06-17T12:05:22Z
dc.date.available2015-06-17T12:05:22Z
dc.date.issued2014
dc.identifier.citationMENEZES, Francisca Gleire Rodrigues de; et al. Detection of virulence genes in environmental strains of Vibrio cholerae from Estuaries in Northeastern Brasil.Rev Inst Med Trop Sao Paulo. v.56, n.5, p.427-32, Sept. Oct. 2014.
dc.identifier.issn0036-4665
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/10872
dc.language.isoeng
dc.publisherInstituto de Medicina Tropical de São Paulo
dc.rightsopen access
dc.titleDetection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazil
dc.typeArticle
dc.identifier.doi10.1590/S0036-46652014000500010
dc.description.abstractenThe objectives of this study were to detect the presence of Vibrio cholerae in tropical estuaries (Northeastern Brazil) and to search for virulence factors in the environmental isolates. Water and sediment samples were inoculated onto a vibrio-selective medium (TCBS), and colonies with morphological resemblance to V. cholerae were isolated. The cultures were identified phenotypically using a dichotomous key based on biochemical characteristics. The total DNA extracted was amplified by PCR to detect ompW and by multiplex PCR to detect the virulence genes ctx, tcp, zot and rfbO1. The results of the phenotypic and genotypic identification were compared. Nine strains of V. cholerae were identified phenotypically, five of which were confirmed by detection of the species-specific gene ompW. The dichotomous key was efficient at differentiating environmental strains of V. cholerae. Strains of V. cholerae were found in all four estuaries, but none possessed virulence genes.
dc.creator.affilliationUniversidade Federal do Ceará. Departamento de Engenharia da Pesca. Fortaleza, CE, Brasil.
dc.creator.affilliationUniversidade Federal do Ceará. Departamento de Engenharia da Pesca. Fortaleza, CE, Brasil.
dc.creator.affilliationUniversidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.
dc.creator.affilliationUniversidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.
dc.creator.affilliationUniversidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.
dc.subject.enCholera
dc.subject.enEstuaries
dc.subject.enPathogenicity
dc.subject.enGenes
dc.subject.decsCólera
dc.subject.decsVirulência
dc.subject.decsGenes
dc.subject.decsEstuários
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