Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11021
Title: Evaluation of the use of C-terminal part of the Schistosoma mansoni 200 kDa tegumental protein in schistosomiasis diagnosis and vaccine formulation
Authors: Carvalho, Gardênia Braz Figueiredo de
Pacífico, Lucila Gonçalves Grossi
Pimenta, Deborah Laranjeira Ferreira
Siqueira, Liliane Maria Vidal
Carvalho, Andréa Teixeira
Coelho, Paulo Marcos Zech
Pinheiro, Carina da Silva
Fujiwara, Ricardo Toshio
Oliveira, Sergio Costa
Fonseca, Cristina Toscano
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
Universidade Federal da Bahia. Departamento de Biointeração do Instituto de Ciências da Saúde. Salvador, BA, Brazil
Universidade Federal de Minas Gerais. Departamento de Bioquímica e imunologia. Laboratório de Imunologia de Doenças Infecciosas. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Imunologia e Genômica de Parasitos. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Instituto Nacional de Ciências e Tecnologia em Doenças Tropicais. Brazil
Abstract: Schistosoma mansoni tegument is involved in essential functions for parasite survival and represents a target for screening candidates for vaccine and diagnosis. Our group using reverse vaccinology selected six candidates, previously demonstrated by proteomics studies to be expressed in the parasite tegument, among them was Sm200. In this work we have cloned and expressed a recombinant form of Sm200 C-terminal (1069-1520) region. The efficacy of rSm200 (1069-1520) in the diagnosis of schistosomiasis and in the formulation of a vaccine against S. mansoni was assessed respectively in an ELISA based diagnostic assay and immunization protocols in mice. Significant differences between non-infected and acutely infected or chronically infected animals were observed and no cross-recognition was observed with sera from Ascaris suum or Ancylostoma ceylanicum infected mice. rSm200-ELISA test could also discriminate infected individuals from healthy donors not living in endemic area for schistosomiasis but failed to discriminate between individuals from a low endemic area for schistosomiasis known to have positive or negative stools after examination. Recombinant Sm200 also failed to induce protection against schistosomiasis, demonstrating that the C-terminal part of Sm200 is unable to induce protective immune response in mice. Therefore rSm200 (1069-1520)-ELISA represents an important tool to be used in the diagnosis of schistosomiasis.
Keywords: Schistosoma mansoni
Tegument
Sm200
Diagnosis
Vaccine
Issue Date: 2014
Publisher: Elsevier Inc.
Citation: CARVALHO, Gardênia Braz Figueiredo de et al. Evaluation of the use of C-terminal part of the Schistosoma mansoni 200 kDa tegumental protein in schistosomiasis diagnosis and vaccine formulation. Exp Parasitol., vol. 139, p. 24-32, 2014
DOI: 10.1016/j.exppara.2014.02.003
ISSN: 0014-4894
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
2014_009.pdf1.95 MBAdobe PDF    Request a copy



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.