| Author | Carvalho, Gardênia Braz Figueiredo de | |
| Author | Pacífico, Lucila Grossi Gonçalves | |
| Author | Pimenta, Deborah Laranjeira Ferreira | |
| Author | Siqueira, Liliane Maria Vidal | |
| Author | Carvalho, Andréa Teixeira | |
| Author | Coelho, Paulo Marcos Zech | |
| Author | Pinheiro, Carina da Silva | |
| Author | Fujiwara, Ricardo Toshio | |
| Author | Oliveira, Sergio Costa | |
| Author | Fonseca, Cristina Toscano | |
| Access date | 2015-06-30T17:33:23Z | |
| Available date | 2015-06-30T17:33:23Z | |
| Document date | 2014 | |
| Citation | CARVALHO, Gardênia Braz Figueiredo de et al. Evaluation of the use of C-terminal part of the Schistosoma mansoni 200 kDa tegumental protein in schistosomiasis diagnosis and vaccine formulation. Exp Parasitol., vol. 139, p. 24-32, 2014 | pt_BR |
| ISSN | 0014-4894 | |
| URI | https://www.arca.fiocruz.br/handle/icict/11021 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Inc. | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Evaluation of the use of C-terminal part of the Schistosoma mansoni 200 kDa tegumental protein in schistosomiasis diagnosis and vaccine formulation | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.exppara.2014.02.003 | |
| Abstract | Schistosoma mansoni tegument is involved in essential functions for parasite survival and represents a target for screening candidates for vaccine and diagnosis. Our group using reverse vaccinology selected six candidates, previously demonstrated by proteomics studies to be expressed in the parasite tegument, among them was Sm200. In this work we have cloned and expressed a recombinant form of Sm200 C-terminal (1069-1520) region. The efficacy of rSm200 (1069-1520) in the diagnosis of schistosomiasis and in the formulation of a vaccine against S. mansoni was assessed respectively in an ELISA based diagnostic assay and immunization protocols in mice. Significant differences between non-infected and acutely infected or chronically infected animals were observed and no cross-recognition was observed with sera from Ascaris suum or Ancylostoma ceylanicum infected mice. rSm200-ELISA test could also discriminate infected individuals from healthy donors not living in endemic area for schistosomiasis but failed to discriminate between individuals from a low endemic area for schistosomiasis known to have positive or negative stools after examination. Recombinant Sm200 also failed to induce protection against schistosomiasis, demonstrating that the C-terminal part of Sm200 is unable to induce protective immune response in mice. Therefore rSm200 (1069-1520)-ELISA represents an important tool to be used in the diagnosis of schistosomiasis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Departamento de Biointeração do Instituto de Ciências da Saúde. Salvador, BA, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e imunologia. Laboratório de Imunologia de Doenças Infecciosas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Imunologia e Genômica de Parasitos. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Instituto Nacional de Ciências e Tecnologia em Doenças Tropicais. Brazil | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | Tegument | pt_BR |
| Subject | Sm200 | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Vaccine | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
