Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11474
Title: Phylogeography and evolutionary history of hepatitis B virus genotype F in Brazil
Authors: Mello, Francisco C. A.
Araujo, Oscar C.
Lago, Barbara V.
Castro, Ana Rita C. Motta
Moraes, Marcia Terezinha B.
Gomes, Selma A.
Bello, Gonzalo
Araujo, Natalia M.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
Universidade Federal de Mato Grosso do Sul. Departamento de Bioquímica e Farmácia. Campo Grande, MS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract: Background: Hepatitis B virus (HBV) genotype F (HBV/F) is considered to be indigenous to the Americas, but its emergence and spread in the continent remain unknown. Previously, only two HBV/F complete genome sequences from Brazil were available, limiting the contribution of Brazilian isolates to the phylogenetic studies of HBV/F. The present study was carried out to assess the proportion and geographic distributions of HBV/F subgenotypes in Brazil, to determine the full-length genomic sequences of HBV/F isolates from different Brazilian geographic regions, and to investigate the detailed evolutionary history and phylogeography of HBV/F in Brazil. Methods: Complete HBV/F genomes isolated from 12 Brazilian patients, representing the HBV/F subgenotypes circulating in Brazil, were sequenced and analyzed together with sequences retrieved from GenBank, using the Bayesian coalescent and phylogeographic framework. Results: Phylogenetic analysis using all Brazilian HBV/F S-gene sequences available in GenBank showed that HBV/F2a is found at higher frequencies countrywide and corresponds to all sequences isolated in the Brazilian Amazon Basin. In addition, the evolutionary analysis using complete genome sequences estimated an older median ancestral age for the Brazilian HBV/F2a compared to the Brazilian HBV/F1b and HBV/F4 subgenotypes, suggesting that HBV/F2a represents the original native HBV of Brazil. The phylogeographic patterns suggested a north-to-south flow of HBV/F2a from Venezuela to Brazil, whereas HBV/F1b and HBV/F4 strains appeared to have spread from Argentina to Brazil. Conclusions: This study suggests a plausible route of introduction of HBV/F subgenotypes in Brazil and demonstrates the usefulness of recently developed computational tools for investigating the evolutionary history of HBV.
Keywords: Hepatitis B Virus
Genotype F
Bayesian framework
Phylogeography
Brazil
keywords: Brasil
DeCS: Filogeografia
Teorema de Bayes
Vírus da Hepatite B
Genótipo
Issue Date: 2013
Publisher: BioMed Central
Citation: MELLO, Francisco C. A.; et al. Phylogeography and evolutionary history of hepatitis B virus genotype F in Brazil. Virology Journal, v.10:236, 8p, 2013.
DOI: 10.1186/1743-422X-10-236
ISSN: 1743-422X
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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