Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/11542
Title: Metabolic Signatures of Triatomine Vectors of Trypanosoma cruzi Unveiled by Metabolomics
Authors: Antunes, Luis Caetano M.
Han, Jun
Pan, Jingxi
Moreira, Carlos J. C.
Azambuja, Patrícia
Borchers, Christoph H.
Carels, Nicolas
Affilliation: The University of British Columbia. Michael Smith Laboratories. Vancoucer, Canada / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para a Inovação em Doenças Negligenciadas. Escola Nacional de Saúde Pública Sérgio Arouca. Rio de Janeiro, RJ, Brasil.
University of Victoria. Genome BC Proteomics Centre. Victoria, Canada.
University of Victoria. Genome BC Proteomics Centre. Victoria, Canada.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT/ME, CNPq). Brasil.
University of Victoria. Genome BC Proteomics Centre. Victoria, Canada.
Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para a Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil / University of Victoria. Genome BC Proteomics Centre. Victoria, Canada / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformàtica. Rio de Janeiro, RJ, Brasil.
Abstract: Chagas disease is a trypanosomiasis whose causative agent is the protozoan parasite Trypanosoma cruzi, which is transmitted to humans by hematophagous insects known as triatomines and affects a large proportion of South America. The digestive tract of the insect vectors in which T. cruzi develops constitutes a dynamic environment that affects the development of the parasite. Thus, we set out to investigate the chemical composition of the triatomine intestinal tract through a metabolomics approach. We performed Direct Infusion Fourier Transform Ion Cyclotron Resonance Mass Spectrometry on fecal samples of three triatomine species (Rhodnius prolixus, Triatoma infestans, Panstrongylus megistus) fed with rabbit blood. We then identified groups of metabolites whose frequencies were either uniform in all species or enriched in each of them. By querying the Human Metabolome Database, we obtained putative identities of the metabolites of interest. We found that a core group of metabolites with uniform frequencies in all species represented approximately 80% of the molecules detected, whereas the other 20% varied among triatomine species. The uniform core was composed of metabolites of various categories, including fatty acids, steroids, glycerolipids, nucleotides, sugars, and others. Nevertheless, the metabolic fingerprint of triatomine feces differs depending on the species considered. The variable core was mainly composed of prenol lipids, amino acids, glycerolipids, steroids, phenols, fatty acids and derivatives, benzoic acid and derivatives, flavonoids, glycerophospholipids, benzopyrans, and quinolines. Triatomine feces constitute a rich and varied chemical medium whose constituents are likely to affect T. cruzi development and infectivity. The complexity of the fecal metabolome of triatomines suggests that it may affect triatomine vector competence for specific T. cruzi strains. Knowledge of the chemical environment of T. cruzi in its invertebrate host is likely to generate new ways to understand the factors influencing parasite proliferation as well as methods to control Chagas disease.
Keywords: Trypanosoma cruzi
Chagas Disease
Triatomine vectors
DeCS: Trypanosoma cruzi
Doença de Chagas
Triatominae
Vetores
Issue Date: 2013
Publisher: Plos One
Citation: ANTUNES, Luis Caetano M.; et al. Metabolic Signatures of Triatomine Vectors of Trypanosoma cruzi Unveiled by Metabolomics. PLoS ONE, v.8, n.10, e77283, 12p, oct. 2013.
DOI: 10.1371/journal.pone.0077283
Copyright: open access
Appears in Collections:CDTS - Artigos de Periódicos
IOC - Artigos de Periódicos

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