Author | Pinheiro, Carla Monica | |
Author | Duarte, Erica dos Santos Martins | |
Author | Ferraro, Rodrigo Barbosa | |
Author | Souza, André Luíz Fonseca de | |
Author | Gomes, Marta T | |
Author | Lopes, Angela H.C.S | |
Author | Santos, Marcos André Vannier dos | |
Author | Santos, André L.S | |
Author | Fernandes, José Roberto Meyer | |
Access date | 2015-09-29T16:08:29Z | |
Available date | 2015-09-29T16:08:29Z | |
Document date | 2006 | |
Citation | PINHEIRO, C. M. et al. Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities. Experimental Parasitology, v. 114, n. 1, p. 16-25, 2006. | pt_BR |
ISSN | 0014-4894 | |
URI | https://www.arca.fiocruz.br/handle/icict/11842 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | Leishmania amazonensis: Biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.exppara.2006.02.007 | |
Abstract | The presence of Leishmania amazonensis ecto-nucleoside triphosphate triphosphohydrolase activities was demonstrated using antibodies against different NTPDase members by Western blotting, flow cytometry, and immunoelectron microscopy analysis. Living promastigote cells sequentially hydrolyzed the ATP molecule generating ADP, AMP, and adenosine, indicating that this surface enzyme may play a role in the salvage of purines from the extracellular medium. The L. amazonensis ecto-NTPDase activities were insensitive to Triton X-100, but they were enhanced by divalent cations, such as Mg(2+). In addition, the ecto-NTPDase activities decreased with time for 96 h when promastigotes were grown in vitro. On the other hand, these activities increased considerably when measured in living amastigote forms. Furthermore, the treatment with adenosine, a mediator of several relevant biological phenomena, induced a decrease in the reactivity with anti-CD39 antibody, raised against mammalian E-NTPDase, probably because of down regulation in the L. amazonensis ecto-NTPDase expression. Also, adenosine and anti-NTPDase antibodies induced a significant diminishing in the interaction between promastigotes of L. amazonensis and mouse peritoneal macrophages. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil | pt_BR |
Subject | Leishmania amazonensis | pt_BR |
Subject | Ecto-ATPase | pt_BR |
Subject | Adenosine | pt_BR |
Subject | E-NTPDase | pt_BR |
DeCS | Adenosina Trifosfatases/metabolismo | pt_BR |
DeCS | Leishmania mexicana/enzimologia | pt_BR |
DeCS | Difosfato de Adenosina/metabolismo | pt_BR |
DeCS | Monofosfato de Adenosina/metabolismo | pt_BR |
DeCS | Trifosfato de Adenosina/metabolismo | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Antígenos CD/metabolismo | pt_BR |
DeCS | Apirase/metabolismo | pt_BR |
DeCS | Western Blotting | pt_BR |
DeCS | Feminino | pt_BR |
DeCS | Citometria de Fluxo | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Leishmaniose Cutânea/parasitologia | pt_BR |
DeCS | Macrófagos Peritoneais/parasitologia | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Microscopia Imunoeletrônica | pt_BR |
DeCS | Pirofosfatases/metabolismo | pt_BR |