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dc.contributor.authorBomfim, Maria Rosa Quaresma
dc.contributor.authorKo, Albert Icksang
dc.contributor.authorKoury, Matilde Cota
dc.identifier.citationBOMFIM, M. R. Q.; KO, A. I.; KOURY, M. C. Evaluation of the recombinant LipL32 in enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis. Veterinary Microbiology, v. 109, n. 1-2, p. 89-94, 2005.
dc.rightsopen access
dc.titleEvaluation of the recombinant LipL32 in enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis.
dc.description.abstractenThe recombinant leptospiral protein LipL32 was evaluated for use in the diagnosis of bovine leptospirosis by enzyme-linked immunosorbent assay (rLipL32 IgG ELISA). The microscopic agglutination test (MAT) of 150 serum samples from cattle suspected of leptospirosis showed that 125 (83.3%) samples had positive reciprocal agglutination titres, which ranged from 100 to 1600. The highest titres were observed for the serovars Hardjoprajitno and Bratislava. In the rLipL32 IgG ELISA, 83.3% of the samples were positive. The sensitivity of IgG ELISA for 125 bovine sera, which had MAT titres of greater than or equal to 100, was 100%. ELISA showed a specificity of 100% with 58 bovine sera, which were negative at a 1:50 dilution in the MAT for Leptospira interrogans serovars. When analytical specificity of the IgG ELISA was evaluted using 60 bovine serum samples from animals showing serum antibodies to other pathogens that cause abortion in cattle, such as Babesia sp., Anaplasma sp. and Brucella sp. and no cross-reaction was observed. The recombinant LipL32 IgG ELISA can be an alternative to the MAT for diagnosis of leptospiral infection in cattle.
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
dc.creator.affilliationWeill Medical College of Cornell University. Division of International Medicine and Infectious Diseases. New York, USA
dc.subject.enrLipL32 IgG ELISA
dc.subject.decsProteínas da Membrana Bacteriana Externa/química
dc.subject.decsDoenças dos Bovinos/microbiologia
dc.subject.decsEnsaio de Imunoadsorção Enzimática/veterinária
dc.subject.decsLeptospira/isolamento & purificação
dc.subject.decsTestes de Aglutinação/veterinária
dc.subject.decsAnticorpos Antibacterianos/sangue
dc.subject.decsDoenças dos Bovinos/sangue
dc.subject.decsEnsaio de Imunoadsorção Enzimática/métodos
dc.subject.decsProteínas Recombinantes/química
dc.subject.decsSensibilidade e Especificidade
Appears in Collections:BA - IGM - Artigos de Periódicos

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