| Author | Camargo, Anderson Carlos | |
| Author | Dias, Mariane Rezende | |
| Author | Cossi, Marcus Vinícius Coutinho | |
| Author | Lanna, Frederico Germano Piscitelli Alvarenga | |
| Author | Cavicchioli, Valéria Quintana | |
| Author | Vallim, Deyse Christina | |
| Author | Pinto, Paulo Sérgio de Arruda | |
| Author | Hofer, Ernesto | |
| Author | Nero, Luís Augusto | |
| Access date | 2015-11-03T16:23:06Z | |
| Available date | 2016-04-01T07:30:09Z | |
| Document date | 2015 | |
| Citation | CAMARGO, Anderson Carlos; et al. Serotypes and Pulsotypes Diversity of Listeria monocytogenes in a Beef-Processing Environment. Foodborne Pathogens and Disease, v.12, n.4, p.323-326, Apr. 2015. | pt_BR |
| ISSN | 1535-3141 | |
| URI | https://www.arca.fiocruz.br/handle/icict/12111 | |
| Language | eng | pt_BR |
| Publisher | Mary Ann Liebert, Inc. | pt_BR |
| Rights | open access | |
| Title | Serotypes and Pulsotypes Diversity of Listeria monocytogenes in a Beef-Processing Environment | pt_BR |
| Type | Article | |
| DOI | 10.1089/fpd.2014.1875 | |
| Abstract | Utensils and equipment from meat-processing facilities are considered relevant cross-contamination points of
Listeria monocytogenes to foods, demanding tracking studies to identify their specific origins, and predict
proper control. The present study aimed to detect L. monocytogenes in a beef-processing facility, investigating
the diversity of serotypes and pulsotypes in order to identify the possible contamination routes. Surface samples
from knives (n = 26), tables (n = 78), and employees hands (n = 74) were collected before and during the
procedures from a beef-processing facility, in addition to surface samples of end cuts: round (n = 32), loin
(n = 30), and chuck (n = 32). All samples were subjected to L. monocytogenes screening according ISO 11.290-
1, and the obtained isolates were subjected to serotyping and pulsed-field gel electrophoresis. Listeria spp. were
identified in all processing steps, in 61 samples, and L. monocytogenes was detected in 17 samples, not being
found only in knives. Eighty-five isolates were identified as L. monocytogenes, from serotypes 1/2c (n = 65), 4b
(n = 13), and 1/2b (n = 7), being grouped in 19 pulsotypes. Considering these results, cross-contamination
among hands, tables, and beef cuts could be identified. The obtained data indicated the relevance of crosscontamination
in the beef-processing facility, and the occurrence of serotypes 1/2b and 4b in beef cuts distributed
for retail sale is a public health concern. | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. | pt_BR |
| Affilliation | undação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. | pt_BR |
| Subject | Listeria monocytogenes | pt_BR |
| Subject | Listeriosis | pt_BR |
| Subject | Cross-contamination | pt_BR |
| Subject | Foodborne diseases | pt_BR |
| DeCS | Doenças Transmitidas por Alimentos | pt_BR |
| DeCS | Listeria monocytogenes | pt_BR |
| DeCS | Listeriose | pt_BR |
| DeCS | Contaminação de Alimentos | pt_BR |
| e-ISSN | 1556-7125 | |
| Embargo date | 2016-03-31 | |
