Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12126
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dc.contributor.authorLima, Estela de Oliveira
dc.contributor.authorMacedo, Cristiana Santos de
dc.contributor.authorEsteves, Cibele Zanardi
dc.contributor.authorOliveira, Diogo Noin de
dc.contributor.authorPessolani, Maria Cristina Vidal
dc.contributor.authorNery, José Augusto da Costa
dc.contributor.authorSarno, Euzenir Nunes
dc.contributor.authorCatharino, Rodrigo Ramos
dc.date.accessioned2015-11-04T14:41:43Z
dc.date.available2016-03-01T07:30:08Z
dc.date.issued2015
dc.identifier.citationLIMA, Estela de Oliveira; et al. Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry. Analytical Chemistry, v.87, p.3585−3592, 2015.
dc.identifier.issn0003-2700
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/12126
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.rightsrestricted access
dc.subject.otherMetodologia de diagnóstico potencial para Hanseníase
dc.titleSkin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry
dc.typeArticle
dc.identifier.doi10.1021/acs.analchem.5b00097
dc.description.abstractenLeprosy is a chronic infectious disease caused by Mycobacterium leprae, which primarily infects macrophages and Schwann cells, affecting skin and peripheral nerves. Clinically, the most common form of identification is through the observation of anesthetic lesions on skin; however, up to 30% of infected patients may not present this clinical manifestation. Currently, the gold standard diagnostic test for leprosy is based on skin lesion biopsy, which is invasive and presents low sensibility for suspect cases. Therefore, the development of a fast, sensible and noninvasive method that identifies infected patients would be helpful for assertive diagnosis. The aim of this work was to identify lipid markers in leprosy patients directly from skin imprints, using a mass spectrometric analytical strategy. For skin imprint samples, a 1 cm2 silica plate was gently pressed against the skin of patients or healthy volunteers. Imprinted silica lipids were extracted and submitted to direct-infusion electrospray ionization high-resolution mass spectrometry (ESI-HRMS). All samples were differentiated using a lipidomics-based data workup employing multivariate data analysis, which helped electing different lipid markers, for example, mycobacterial mycolic acids, inflammatory and apoptotic molecules were identified as leprosy patients’ markers. Otherwise, phospholipids and gangliosides were pointed as healthy volunteers’ skin lipid markers, according to normal skin composition. Results indicate that silica plate skin imprinting associated with ESI-HRMS is a promising fast and sensible leprosy diagnostic method. With a prompt leprosy diagnosis, an early and effective treatment could be feasible and thus the chain of leprosy transmission could be abbreviated.
dc.creator.affilliationUniversidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Desenvolvimento Tecnólogico em Saúde (CDTS). Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.
dc.creator.affilliationUniversidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Ambulatório Souza Araújo. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade de Campinas. Escola de Ciências Farmacêuticas. Laboratório Innovare de Biomarcadores. Campinas, SP, Brasil.
dc.subject.enLeprosy
dc.subject.enHigh-Resolution Electrospray Mass Spectrometry (ESIHRMS)
dc.subject.enSkin Imprinting in Silica Plates
dc.subject.enPotential Diagnostic Methodology for Leprosy
dc.subject.decsHanseníase
dc.subject.decsEspectrometria de Massas
dc.subject.decsPlacas de sílica
Appears in Collections:CDTS - Artigos de Periódicos
IOC - Artigos de Periódicos

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