Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/12298
Title: Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans.
Authors: Murray, Gerald L
Srikram, Amporn
Hoke, David E
Wunder Junior, Elsio Augusto
Henry, Rebekah
Lo, Miranda
Zhang, Kunkun
Sermswan, Rasana W
Ko, Albert Icksang
Adler, Ben
Affilliation: Monash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, Australia
Khon Kaen University. Faculty of Medicine. Melioidosis Research Center. Department of Biochemistry. Khon Kaen, Thailand
Monash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, Australia
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Monash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, Australia
Monash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, Australia
Monash University. Functional Microbial Genomics. Department of Microbiology. Clayton, Australia
Khon Kaen University. Faculty of Medicine. Khon Kaen, Thailand
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. New York, New York
Monash University. Australian Bacterial Pathogenesis Program / Australian Research Council Centre of Excellence in Structural. Clayton, Australia / Monash University. Functional Microbial Genomics. Department of Microbiology. Clayton, Australia
Abstract: Leptospira interrogans is responsible for leptospirosis, a zoonosis of worldwide distribution. LipL32 is the major outer membrane protein of pathogenic leptospires, accounting for up to 75% of total outer membrane protein. In recent times LipL32 has become the focus of intense study because of its surface location, dominance in the host immune response, and conservation among pathogenic species. In this study, an lipL32 mutant was constructed in L. interrogans using transposon mutagenesis. The lipL32 mutant had normal morphology and growth rate compared to the wild type and was equally adherent to extracellular matrix. Protein composition of the cell membranes was found to be largely unaffected by the loss of LipL32, with no obvious compensatory increase in other proteins. Microarray studies found no obvious stress response or upregulation of genes that may compensate for the loss of LipL32 but did suggest an association between LipL32 and the synthesis of heme and vitamin B12. When hamsters were inoculated by systemic and mucosal routes, the mutant caused acute severe disease manifestations that were indistinguishable from wild-type L. interrogans infection. In the rat model of chronic infection, the LipL32 mutant colonized the renal tubules as efficiently as the wild-type strain. In conclusion, this study showed that LipL32 does not play a role in either the acute or chronic models of infection. Considering the abundance and conservation of LipL32 among all pathogenic Leptospira spp. and its absence in saprophytic Leptospira, this finding is remarkable. The role of this protein in leptospiral biology and pathogenesis thus remains elusive.
DeCS: Proteínas da Membrana Bacteriana Externa/metabolismo
Leptospira interrogans/patogenicidade
Leptospirose/metabolismo
Lipoproteínas/metabolismo
Animais
Proteínas da Membrana Bacteriana Externa/genética
Western Blotting
Cricetinae
Leptospira interrogans/genética
Leptospira interrogans/metabolismo
Leptospirose/genética
Mesocricetus
Análise de Sequência com Séries de Oligonucleotídeos
Reação em Cadeia da Polimerase
Ratos
Ratos Wistar
Issue Date: 2009
Publisher: American Society of Microbiology
Citation: MURRAY, G. L. et al. Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans. Infection and Immunity, v. 77, n. 3, p. 952-958, 2009.
DOI: 10.1128/IAI.01370-08
ISSN: 1098-5522
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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